Li Jianrui, Dong Yang, Li Cong, Pan Yanlin, Yu Jingjuan
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University Beijing, China.
Front Plant Sci. 2017 Jan 12;7:2053. doi: 10.3389/fpls.2016.02053. eCollection 2016.
Drought and other types of abiotic stresses negatively affect plant growth and crop yields. The abscisic acid-, stress-, and ripening-induced (ASR) proteins play important roles in the protection of plants against abiotic stress. However, the regulatory pathway of the gene encoding this protein remains to be elucidated. In this study, the foxtail millet () ASR gene, , was cloned and characterized. SiASR4 localized to the cell nucleus, cytoplasm and cytomembrane, and the protein contained 102 amino acids, including an ABA/WDS (abscisic acid/water-deficit stress) domain, with a molecular mass of 11.5 kDa. The abundance of transcripts increased after treatment with ABA, NaCl, and PEG in foxtail millet seedlings. It has been reported that the ABA-responsive DRE-binding protein (SiARDP) binds to a DNA sequence with a CCGAC core and that there are five dehydration-responsive element (DRE) motifs within the promoter. Our analyses demonstrated that the SiARDP protein could bind to the promoter and . The expression of increased in -overexpressing plants. -transgenic and -overexpressing foxtail millet exhibited enhanced tolerance to drought and salt stress. Furthermore, the transcription of stress-responsive and reactive oxygen species (ROS) scavenger-associated genes was activated in transgenic plants. Together, these findings show that functions in the adaption to drought and salt stress and is regulated by via an ABA-dependent pathway.
干旱和其他类型的非生物胁迫会对植物生长和作物产量产生负面影响。脱落酸、胁迫和成熟诱导（ASR）蛋白在保护植物免受非生物胁迫方面发挥着重要作用。然而，编码该蛋白的基因的调控途径仍有待阐明。在本研究中，对谷子（）ASR基因进行了克隆和表征。SiASR4定位于细胞核、细胞质和细胞膜，该蛋白包含102个氨基酸，包括一个ABA/WDS（脱落酸/水分亏缺胁迫）结构域，分子量为11.5 kDa。在谷子幼苗中用ABA、NaCl和PEG处理后，转录本的丰度增加。据报道，ABA响应性DRE结合蛋白（SiARDP）与具有CCGAC核心的DNA序列结合，并且在启动子内有五个脱水响应元件（DRE）基序。我们的分析表明，SiARDP蛋白可以与启动子和结合。在过表达植物中表达增加。转基因和过表达谷子对干旱和盐胁迫表现出增强的耐受性。此外，在转基因植物中，胁迫响应和活性氧（ROS）清除剂相关基因的转录被激活。总之，这些发现表明在适应干旱和盐胁迫中起作用，并通过ABA依赖途径受调控。
