伊朗德黑兰省废水样本中[具体物质]的微观与分子检测
Microscopic and Molecular Detection of and in Wastewater Samples of Tehran Province, Iran.
作者信息
Hatam-Nahavandi Kareem, Mohebali Mehdi, Mahvi Amir-Hossein, Keshavarz Hossein, Najafian Hamid-Reza, Mirjalali Hamed, Rezaei Sasan, Rezaeian Mostafa
机构信息
Department of Parasitology and Mycology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran; Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Parasitol. 2016 Oct-Dec;11(4):499-506.
BACKGROUND
As a waterborne pathogen, is one of the most common causes of gastroenteritis in human and hoofed livestock animals. This study aimed to investigate the distribution of spp. in human and livestock wastewaters in Iran, by the 18S rRNA sequence analysis.
METHODS
A total of 54 raw wastewater samples collected from three urban treatment plants and two slaughterhouses during 2014-2015 in Tehran, Iran. The presence of the oocysts was assessed by immunofluorescence with monoclonal antibodies. To characterize the oocysts at the molecular level, the 18S rRNA gene of was PCR amplified and sequenced.
RESULTS
Of the 54 wastewater samples examined, 34 (62.9%) were positive for oocysts using the IFA. Of these, 70.5% (24/34) were positive by PCR, that 91.6% (22/24) were successfully sequenced. The species of (95.4%) and (4.6%) were detected in livestock wastewater samples.
CONCLUSION
was the major sp. found in the aquatic environmental wastewater samples. The high rate of detection of in domestic wastewater was probably the result of the predominancy of this species in cattle herds in Iran. The current study is the first report of in Iran.
背景
作为一种水源性病原体，是人类和有蹄类家畜肠胃炎最常见的病因之一。本研究旨在通过18S rRNA序列分析调查伊朗人类和家畜废水中该病原体的分布情况。
方法
2014年至2015年期间，从伊朗德黑兰的三个城市污水处理厂和两个屠宰场共采集了54份原废水样本。通过单克隆抗体免疫荧光法评估该病原体卵囊的存在情况。为了在分子水平上鉴定卵囊，对该病原体的18S rRNA基因进行PCR扩增和测序。
结果
在所检测的54份废水样本中，使用免疫荧光法检测到34份（62.9%）该病原体卵囊呈阳性。其中，70.5%（24/34）通过PCR检测呈阳性，91.6%（22/24）成功测序。在家畜废水样本中检测到该病原体（95.4%）和另一种（4.6%）。
结论
该病原体是在水生环境废水样本中发现的主要病原体种类。生活污水中该病原体的高检出率可能是由于其在伊朗牛群中占主导地位所致。本研究是伊朗关于该病原体的首次报道。
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