Dossi Camila G, Cadagan Cynthia, San Martín Marcela, Espinosa Alejandra, González-Mañán Daniel, Silva David, Mancilla Rodrigo A, Tapia Gladys S
Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
Department of Medical Technology, Faculty of Medicine, University of Chile, Santiago, Chile.
Food Funct. 2017 Feb 22;8(2):832-841. doi: 10.1039/c6fo01762b.
Rosa mosqueta (RM) oil is rich in α-linolenic acid (ALA) - a precursor of eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), and it has a high antioxidant activity due to its abundant content of tocopherols. Additionally, it has been observed that RM oil administration prevents hepatic steatosis. Thus, the aim of this study was to demonstrate the antilipogenic mechanism related to RM oil administration in a high-fat diet (HFD) fed mice model by evaluating markers associated with the regulation of lipid droplet metabolism (PLIN2, PLIN5 and PPAR-γ), and proteins associated with lipogenesis (FAS and SREBP-1c). C57BL/6J mice were fed either a control diet or a HFD, with and without RM oil supplementation for 12 weeks. The results showed that RM oil supplementation decreases hepatic PLIN2 and PPAR-γ mRNA expression and SREBP-1c, FAS and PLIN2 protein levels, whereas we did not find changes in the level of PLIN5 among the groups. These results suggest that modulation of lipogenic markers could be one of the mechanisms, through which RM oil supplementation prevents the hepatic steatosis induced by HFD consumption in a mice model.
玫瑰果（RM）油富含α-亚麻酸（ALA）——二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的前体，并且由于其生育酚含量丰富而具有高抗氧化活性。此外，据观察，给予RM油可预防肝脂肪变性。因此，本研究的目的是通过评估与脂滴代谢调节相关的标志物（PLIN2、PLIN5和PPAR-γ）以及与脂肪生成相关的蛋白质（FAS和SREBP-1c），来证明在高脂饮食（HFD）喂养的小鼠模型中与给予RM油相关的抗脂肪生成机制。将C57BL/6J小鼠分为对照组饮食或HFD组，分别给予或不给予RM油补充剂，持续12周。结果显示，补充RM油可降低肝脏中PLIN2和PPAR-γ mRNA表达以及SREBP-1c、FAS和PLIN2蛋白水平，而我们未发现各组间PLIN5水平有变化。这些结果表明，调节脂肪生成标志物可能是补充RM油预防小鼠模型中因食用HFD诱导的肝脂肪变性的机制之一。
