Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
Department of Food Science and Chemical Technology, University of Chile, Santiago, Chile.
Nutrition. 2018 Sep;53:1-8. doi: 10.1016/j.nut.2018.01.012. Epub 2018 Apr 3.
The aim of this study was to evaluate the contribution of tocopherols present in Rosa mosqueta oil (RM) in the prevention of high-fat diet (HFD)-induced alterations.
Male C57 BL/6 J mice (n = 9/group) were fed for 12 wk and divided into four groups: control (CD; 10% kcal fat, 20% kcal protein, 70% kcal carbohydrates); HFD (60% as fat, 20% kcal protein, 20% kcal carbohydrates); HFD + RM (0.01 mL/g body weight/d); and HFD + RM without tocopherols (0.01 mL/g body weight/d). Parameters of obesity, liver steatosis (histology, triacylglycerols content), inflammation (adipose NLRP3 inflammasome, tumor necrosis factor-α and interleukin-1 β expression, hepatic nuclear factor-κB) and oxidative stress (hepatic Nrf2 activation, carbonylated proteins) were evaluated.
Liver steatosis, inflammatory, and oxidative stress parameters were significantly (P < 0.05) increased in the HFD + RM compared with the HFD + RM, with no differences between HFD and HFD + RM.
The present study suggests that α- and γ-tocopherols from RM may have an important role in the prevention of alterations induced by HFD.
本研究旨在评估野玫瑰果油（RM）中生育酚对高脂肪饮食（HFD）诱导的改变的预防作用。
雄性 C57BL/6 J 小鼠（n=9/组）喂养 12 周，并分为四组：对照组（CD；10%卡路里脂肪，20%卡路里蛋白质，70%卡路里碳水化合物）；HFD（60%脂肪，20%卡路里蛋白质，20%卡路里碳水化合物）；HFD+RM（0.01mL/g 体重/天）；和 HFD+不含生育酚的 RM（0.01mL/g 体重/天）。评估肥胖、肝脂肪变性（组织学、三酰甘油含量）、炎症（脂肪组织 NLRP3 炎症小体、肿瘤坏死因子-α 和白细胞介素-1β 表达、肝核因子-κB）和氧化应激（肝 Nrf2 激活、羰基化蛋白）的参数。
与 HFD+RM 相比，HFD+RM 组肝脂肪变性、炎症和氧化应激参数显著增加（P<0.05），但 HFD 组与 HFD+RM 组之间无差异。
本研究表明，RM 中的α-和γ-生育酚可能在预防 HFD 诱导的改变方面发挥重要作用。
