Feng H, Sui L, Du M, Wang Q
Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China
Genet Mol Res. 2017 Jan 23;16(1):gmr-16-01-gmr.16016571. doi: 10.4238/gmr16016571.
The aim of this study was to investigate the tumor protein p73 (TP73) G4C14-A4T14 polymorphism and to perform a meta-analysis to assess TP73 expression in cervical cancer and precancerous tissue. Articles containing data regarding TP73 status in cervical cancer patients and healthy controls were retrieved from PubMed, EMBASE, Cochrane, Chinese Biomedical Literature, and China National Knowledge Infrastructure databases. Then, the quality of the studies was evaluated according to inclusion and exclusion criteria. Odds ratios between the case and control groups were used as an effect evaluation index and the RevMan 5.0 software was employed to carry out the meta-analysis. Three independent investigations including 8452 cases of cervical cancer and 8326 healthy controls were included in our study following the application of inclusion and exclusion criteria. PCR genotyping revealed that the TP73 GC/GC genotype produced a 193-bp product, while the AT/AT genotype produced a 270-bp fragment, and GC/AT genotype samples produced two fragments of 193 and 270 bp. Meta-analysis showed that TP73 expression in cervical cancer was significantly higher than that in normal cervical squamous epithelium (P < 0.05). This elevated expression may play an important role in the occurrence and development of cervical cancer. Therefore, TP73 testing might be useful in the screening and diagnosis of cervical cancer and precancerous lesions.
本研究旨在调查肿瘤蛋白p73（TP73）G4C14-A4T14多态性，并进行荟萃分析以评估TP73在宫颈癌及癌前组织中的表达。从PubMed、EMBASE、Cochrane、中国生物医学文献数据库和中国知网数据库中检索包含宫颈癌患者和健康对照者TP73状态数据的文章。然后，根据纳入和排除标准对研究质量进行评估。病例组与对照组之间的比值比用作效应评估指标，并采用RevMan 5.0软件进行荟萃分析。在应用纳入和排除标准后，本研究纳入了三项独立调查，包括8452例宫颈癌病例和8326例健康对照。聚合酶链反应基因分型显示，TP73 GC/GC基因型产生一个193 bp的产物，而AT/AT基因型产生一个270 bp的片段，GC/AT基因型样本产生193和270 bp的两个片段。荟萃分析表明，宫颈癌中TP73的表达显著高于正常宫颈鳞状上皮（P < 0.05）。这种表达升高可能在宫颈癌的发生和发展中起重要作用。因此，TP73检测可能有助于宫颈癌及癌前病变的筛查和诊断。
