Gao G L, Wang C, Zhao X Z, Wang H W, Li Q, Li J, Zhang K S, Zhong H, Wang Q G
Chongqing Academy of Animal Sciences, Chongqing, China.
Chongqing Academy of Animal Sciences, Chongqing, China
Genet Mol Res. 2017 Jan 23;16(1):gmr-16-01-gmr.16019119. doi: 10.4238/gmr16019119.
Chicken meat quality is becoming increasingly important among breeders and consumers. To understand the effect of feeding conditions on chicken meat quality, we investigated the profiles of genes expressed in chicken breast muscle. Using RNA sequencing, we identified 336, 321, and 387 differentially expressed genes among Chengkou, Daninghe, and Qingjiaoma chickens under scatter- and captivity-feeding conditions. Twenty-two genes differentially expressed between different feeding conditions were shown to be common among the three breeds. Seven of these genes were assessed by real-time quantitative PCR, which confirmed the findings of RNA sequencing and suggested that the results were viable. The differentially expressed genes showed enrichment for a series of significant pathways, including energy metabolism, xenobiotics biodegradation and metabolism, and the immune system. These results provide a solid foundation for elucidating the molecular mechanisms underlying chicken meat quality.
鸡肉品质在养殖者和消费者中变得越来越重要。为了了解饲养条件对鸡肉品质的影响，我们研究了鸡胸肌中基因的表达谱。通过RNA测序，我们在散养和圈养条件下的城口鸡、大宁河鸡和青脚麻鸡中分别鉴定出336、321和387个差异表达基因。在不同饲养条件下差异表达的22个基因在这三个品种中是共有的。其中7个基因通过实时定量PCR进行了评估，这证实了RNA测序的结果，并表明这些结果是可靠的。差异表达基因在一系列重要途径中表现出富集，包括能量代谢、异生物质的生物降解和代谢以及免疫系统。这些结果为阐明鸡肉品质潜在的分子机制提供了坚实的基础。
