Hu Yongfei, Huang Yan, Yi Ying, Wang Hongwei, Liu Bing, Yu Jia, Wang Dong
a College of Bioinformatics Science and Technology , Harbin Medical University , Harbin , China.
b 307-Ivy Translational Medicine Center , Laboratory of Oncology, Affiliated Hospital, Academy of Military Medical Sciences , Beijing , China.
Autophagy. 2017 Apr 3;13(4):770-771. doi: 10.1080/15548627.2016.1278093. Epub 2017 Jan 27.
Accumulating evidence has demonstrated that macroautophagy/autophagy plays an essential role in self-renewal and differentiation in embryonic hematopoiesis. Here, according to the RNA sequencing data sets of 5 population cells related to hematopoietic stem cell (HSC) formation during mouse embryogenesis (endothelial cells, PTPRC/CD45 and PTPRC/CD45 pre-HSCs in the E11 aorta-gonad-mesonephros (AGM) region, mature HSCs in E12 and E14 fetal liver), we explored the dynamic expression of mouse autophagy-related genes in this course at the single-cell level. Our results revealed that the transcription activity of autophagy-related genes had a substantial increase when endothelial cells (ECs) specified into pre-HSCs, and the upregulation of autophagy-essential genes correlated with reduced NOTCH signaling in pre-HSCs, suggesting the autophagy activity may be greatly enhanced during pre-HSC specification from endothelial precursors. In summary, our results presented strong evidence that autophagy plays a critical role in HSC emergence during mouse midgestation.
越来越多的证据表明，巨自噬/自噬在胚胎造血的自我更新和分化中起着至关重要的作用。在此，根据小鼠胚胎发育过程中与造血干细胞（HSC）形成相关的5种群体细胞（内皮细胞、E11主动脉-性腺-中肾（AGM）区域的PTPRC/CD45和PTPRC/CD45前HSC、E12和E14胎肝中的成熟HSC）的RNA测序数据集，我们在单细胞水平上探索了小鼠自噬相关基因在此过程中的动态表达。我们的结果显示，当内皮细胞（EC）分化为前HSC时，自噬相关基因的转录活性大幅增加，并且自噬必需基因的上调与前HSC中NOTCH信号的降低相关，这表明在内皮前体细胞向前HSC分化的过程中，自噬活性可能会大大增强。总之，我们的结果提供了强有力的证据，表明自噬在小鼠妊娠中期HSC的出现中起关键作用。
