Makri Sotiria, Kafantaris Ioannis, Stagos Dimitrios, Chamokeridou Theodora, Petrotos Konstantinos, Gerasopoulos Konstantinos, Mpesios Anastasios, Goutzourelas Nikolaos, Kokkas Stylianos, Goulas Panagiotis, Komiotis Dimitrios, Kouretas Dimitrios
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110, Larissa, Greece.
Food Chem Toxicol. 2017 Apr;102:24-31. doi: 10.1016/j.fct.2017.01.019. Epub 2017 Jan 24.
Currently, there is a great interest in the production of animal feed with antioxidant activity. The aim of this study was to examine the potential antioxidant effects of a feed supplemented with grape pomace (GP), a winery by-product with high environmental load, in chickens. Broilers of 15 days post birth were separated into two groups fed either with standard diet or with diet supplemented with GP for 35 days. Blood and tissues collections were performed after feeding for 15 and 35 days with the experimental diet (i.e. at 30 and 50 days post birth). Free radical toxicity markers, namely thiobarbituric acid reactive substances, protein carbonyls, total antioxidant capacity, reduced glutathione, catalase activity and rate of HO decomposition were determined in blood and tissues of vital organs. The results indicated that feed supplemented with GP decreased oxidative stress-induced toxic effects and improved chickens' redox status, and so it may also improve their wellness and productivity. On the other hand, this exploitation of GP may solve problems of environmental pollution in areas with wineries.
目前,人们对生产具有抗氧化活性的动物饲料有着浓厚的兴趣。本研究的目的是检测添加葡萄皮渣(GP)的饲料对鸡的潜在抗氧化作用,葡萄皮渣是一种具有高环境负荷的酿酒厂副产品。将出生15天的肉鸡分成两组,分别喂食标准日粮或添加GP的日粮,持续35天。在使用实验日粮喂养15天和35天后(即出生后30天和50天)进行血液和组织采集。测定了重要器官血液和组织中的自由基毒性标志物,即硫代巴比妥酸反应性物质、蛋白质羰基、总抗氧化能力、还原型谷胱甘肽、过氧化氢酶活性和HO分解速率。结果表明,添加GP的饲料降低了氧化应激诱导的毒性作用,改善了鸡的氧化还原状态,因此也可能改善它们的健康状况和生产性能。另一方面,对葡萄皮渣的这种利用可能解决酿酒厂所在地区的环境污染问题。