• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

褪黑素-微量营养素治疗骨质减少症研究（MOTS）：一项转化研究，在一项为期一年的双盲随机对照试验后，评估褪黑素、锶（柠檬酸盐）、维生素D3和维生素K2（MK7）对绝经后骨质减少女性的骨密度、骨标志物周转率和健康相关生活质量的影响，以及对成骨细胞-破骨细胞共培养的影响。

Melatonin-micronutrients Osteopenia Treatment Study (MOTS): a translational study assessing melatonin, strontium (citrate), vitamin D3 and vitamin K2 (MK7) on bone density, bone marker turnover and health related quality of life in postmenopausal osteopenic women following a one-year double-blind RCT and on osteoblast-osteoclast co-cultures.

作者信息

Maria Sifat, Swanson Mark H, Enderby Larry T, D'Amico Frank, Enderby Brianna, Samsonraj Rebekah M, Dudakovic Amel, van Wijnen Andre J, Witt-Enderby Paula A

机构信息

Division of Pharmaceutical Sciences, Duquesne University School of Pharmacy, Pittsburgh, PA 15282, USA.

Functional Medicine, Heart Preventics, LLC, Sequim, WA 98382, USA.

出版信息

Aging (Albany NY). 2017 Jan 26;9(1):256-285. doi: 10.18632/aging.101158.

DOI:10.18632/aging.101158
PMID:28130552
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5310667/
Abstract

This one-year double blind randomized control trial assessed the effects of nightly melatonin, strontium (citrate), vitamin D3 and vitamin K2 (MK7; MSDK) on bone mineral density (BMD) and quality of life (QOL) in postmenopausal osteopenic women (ages 49-75). Compared to placebo, MSDK treatment increased BMD in lumbar spine (4.3%) and left femoral neck (2.2%), with an upward trend for total left hip (p=0.069). MSDK increased serum P1NP levels and reduced bone turnover (CTx:P1NP). Psychometric analyses indicated that mood and sleep quality improved for the MSDK group. MSDK-exposed human mesenchymal stem cells (hMSCs) and human peripheral blood monocytes (hPBMCs) plated in transwells or layered demonstrated increases in osteoblastogenesis, decreases in osteoclastogenesis, increases in OPG (TNFRSF11B) and decreases in RANKL (TNFSF11) levels. In transwell osteoblasts, MSDK increased pERK1/2 (MAPK1/MAPK3) and RUNX2 levels; decreased ERK5 (MAPK7); and did not affect the expression of NFκB (NFKB1) and β1integrin (ITGB1). In layered osteoblasts, MSDK also decreased expression of the metabolic proteins PPARγ (PPARG) and GLUT4 (SLC2A4). In adipose-derived human MSCs, MSDK induced osteoblastogenesis. These findings provide both clinical and mechanistic support for the use of MSDK for the prevention or treatment of osteopenia, osteoporosis or other bone-related diseases.

摘要

这项为期一年的双盲随机对照试验评估了夜间服用褪黑素、柠檬酸锶、维生素D3和维生素K2（MK7；MSDK）对绝经后骨质减少女性（49 - 75岁）骨密度（BMD）和生活质量（QOL）的影响。与安慰剂相比，MSDK治疗使腰椎骨密度增加了4.3%，左股骨颈骨密度增加了2.2%，左全髋骨密度有上升趋势（p = 0.069）。MSDK提高了血清I型前胶原氨基端前肽（P1NP）水平并降低了骨转换（I型胶原交联C末端肽：P1NP）。心理测量分析表明，MSDK组的情绪和睡眠质量有所改善。接种于Transwell小室或铺层培养的经MSDK处理的人间充质干细胞（hMSCs）和人外周血单核细胞（hPBMCs）显示成骨细胞生成增加，破骨细胞生成减少，骨保护素（OPG，肿瘤坏死因子受体超家族成员11B）增加，核因子κB受体活化因子配体（RANKL，肿瘤坏死因子配体超家族成员11）水平降低。在Transwell小室培养的成骨细胞中，MSDK增加了磷酸化细胞外信号调节激酶1/2（pERK1/2，丝裂原活化蛋白激酶1/丝裂原活化蛋白激酶3）和RUNX2水平；降低了细胞外信号调节激酶5（ERK5，丝裂原活化蛋白激酶7）；并且不影响核因子κB（NFκB1）和β1整合素（ITGB1）的表达。在铺层培养的成骨细胞中，MSDK还降低了代谢蛋白过氧化物酶体增殖物激活受体γ（PPARγ，PPARG）和葡萄糖转运蛋白4（GLUT4，溶质载体家族2成员4）的表达。在脂肪来源的人间充质干细胞中，MSDK诱导了成骨细胞生成。这些发现为使用MSDK预防或治疗骨质减少、骨质疏松或其他骨相关疾病提供了临床和机制方面的支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/d3e0c7d21620/aging-09-0256-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/53689166180b/aging-09-0256-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/f637a04dfd58/aging-09-0256-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/a468afe9ee9e/aging-09-0256-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/2f4774b12778/aging-09-0256-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/ab8f7f513139/aging-09-0256-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/5d72cb593d96/aging-09-0256-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/03a788b03a24/aging-09-0256-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/c3f72dc5b3f5/aging-09-0256-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/2bd9dc53f458/aging-09-0256-g008b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/c527bd781310/aging-09-0256-g009a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/356b3871c159/aging-09-0256-g009b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/7d50b3dd0a7f/aging-09-0256-g010a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/e592c6d764fd/aging-09-0256-g010b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/219fa946b358/aging-09-0256-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/d3e0c7d21620/aging-09-0256-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/53689166180b/aging-09-0256-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/f637a04dfd58/aging-09-0256-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/a468afe9ee9e/aging-09-0256-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/2f4774b12778/aging-09-0256-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/ab8f7f513139/aging-09-0256-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/5d72cb593d96/aging-09-0256-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/03a788b03a24/aging-09-0256-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/c3f72dc5b3f5/aging-09-0256-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/2bd9dc53f458/aging-09-0256-g008b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/c527bd781310/aging-09-0256-g009a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/356b3871c159/aging-09-0256-g009b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/7d50b3dd0a7f/aging-09-0256-g010a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/e592c6d764fd/aging-09-0256-g010b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/219fa946b358/aging-09-0256-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/5310667/d3e0c7d21620/aging-09-0256-g012.jpg

相似文献

1
Melatonin-micronutrients Osteopenia Treatment Study (MOTS): a translational study assessing melatonin, strontium (citrate), vitamin D3 and vitamin K2 (MK7) on bone density, bone marker turnover and health related quality of life in postmenopausal osteopenic women following a one-year double-blind RCT and on osteoblast-osteoclast co-cultures.褪黑素-微量营养素治疗骨质减少症研究（MOTS）：一项转化研究，在一项为期一年的双盲随机对照试验后，评估褪黑素、锶（柠檬酸盐）、维生素D3和维生素K2（MK7）对绝经后骨质减少女性的骨密度、骨标志物周转率和健康相关生活质量的影响，以及对成骨细胞-破骨细胞共培养的影响。
Aging (Albany NY). 2017 Jan 26;9(1):256-285. doi: 10.18632/aging.101158.
2
Biological effects of melatonin on osteoblast/osteoclast cocultures, bone, and quality of life: Implications of a role for MT2 melatonin receptors, MEK1/2, and MEK5 in melatonin-mediated osteoblastogenesis.褪黑素对成骨细胞/破骨细胞共培养物、骨骼和生活质量的生物学影响：MT2 褪黑素受体、MEK1/2 和 MEK5 在褪黑素介导的成骨作用中的作用意义。
J Pineal Res. 2018 Apr;64(3). doi: 10.1111/jpi.12465. Epub 2018 Jan 17.
3
Melatonin improves bone mineral density at the femoral neck in postmenopausal women with osteopenia: a randomized controlled trial.褪黑素可改善绝经后骨质疏松症女性股骨颈骨密度：一项随机对照试验。
J Pineal Res. 2015 Sep;59(2):221-9. doi: 10.1111/jpi.12252. Epub 2015 Jun 24.
4
Vitamin K supplementation in postmenopausal women with osteopenia (ECKO trial): a randomized controlled trial.绝经后骨质减少女性补充维生素K（ECKO试验）：一项随机对照试验。
PLoS Med. 2008 Oct 14;5(10):e196. doi: 10.1371/journal.pmed.0050196.
5
The effect of vitamin MK-7 on bone mineral density and microarchitecture in postmenopausal women with osteopenia, a 3-year randomized, placebo-controlled clinical trial.维生素 MK-7 对患有骨质疏松症的绝经后妇女骨密度和微结构的影响：一项为期 3 年的随机、安慰剂对照临床试验。
Osteoporos Int. 2021 Jan;32(1):185-191. doi: 10.1007/s00198-020-05638-z. Epub 2020 Oct 8.
6
Effect of low-dose alendronate treatment on bone mineral density and bone turnover markers in Chinese postmenopausal women with osteopenia and osteoporosis.低剂量阿仑膦酸钠治疗对中国绝经后骨质疏松和骨量减少症妇女骨密度和骨转换标志物的影响。
Menopause. 2013 Jan;20(1):72-8. doi: 10.1097/gme.0b013e31825fe2e8.
7
Effect of vitamin K2 treatment on carboxylation of osteocalcin in early postmenopausal women.维生素K2治疗对绝经后早期女性骨钙素羧化作用的影响。
Gynecol Endocrinol. 2006 Aug;22(8):455-9. doi: 10.1080/09513590600900402.
8
Effects of a Multispecies Probiotic Supplement on Bone Health in Osteopenic Postmenopausal Women: A Randomized, Double-blind, Controlled Trial.多菌种益生菌补充剂对骨质疏松绝经后妇女骨骼健康的影响：一项随机、双盲、对照试验。
J Am Coll Nutr. 2017 Sep-Oct;36(7):497-506. doi: 10.1080/07315724.2017.1318724. Epub 2017 Jun 19.
9
Higher levels of s-RANKL and osteoprotegerin in children and adolescents with type 1 diabetes mellitus may indicate increased osteoclast signaling and predisposition to lower bone mass: a multivariate cross-sectional analysis.1型糖尿病儿童和青少年中较高水平的可溶性核因子κB受体活化因子配体（s-RANKL）和骨保护素可能表明破骨细胞信号增加以及骨量降低的易感性：一项多变量横断面分析。
Osteoporos Int. 2016 Apr;27(4):1631-1643. doi: 10.1007/s00198-015-3422-5. Epub 2015 Nov 20.
10
Twelve-month consumption of a polyphenol extract from olive (Olea europaea) in a double blind, randomized trial increases serum total osteocalcin levels and improves serum lipid profiles in postmenopausal women with osteopenia.在一项随机双盲试验中，连续 12 个月摄入橄榄（Olea europaea）多酚提取物可增加骨质疏松症绝经后妇女的血清总骨钙素水平并改善其血脂谱。
J Nutr Health Aging. 2015 Jan;19(1):77-86. doi: 10.1007/s12603-014-0480-x.

引用本文的文献

1
Effects of dietary supplements on bone turnover markers in women after menopause: a network meta-analysis.膳食补充剂对绝经后女性骨转换标志物的影响：一项网状Meta分析。
PeerJ. 2025 Sep 8;13:e19882. doi: 10.7717/peerj.19882. eCollection 2025.
2
Co-Culture Approaches in Cartilage and Bone Tissue Regeneration.软骨和骨组织再生中的共培养方法。
Int J Mol Sci. 2025 Jun 14;26(12):5711. doi: 10.3390/ijms26125711.
3
Melatonin: A Potential Therapy for Osteoporosis With Insights Into Molecular Mechanisms.褪黑素：对骨质疏松症的一种潜在治疗方法及分子机制洞察

本文引用的文献

1
Enhancer of Zeste Homolog 2 Inhibition Stimulates Bone Formation and Mitigates Bone Loss Caused by Ovariectomy in Skeletally Mature Mice.Zeste 同源物 2 抑制因子可刺激骨骼成熟小鼠的骨形成并减轻卵巢切除引起的骨质流失。
J Biol Chem. 2016 Nov 18;291(47):24594-24606. doi: 10.1074/jbc.M116.740571. Epub 2016 Oct 10.
2
p38 MAPK Signaling in Osteoblast Differentiation.p38 MAPK 信号通路在成骨细胞分化中的作用。
Front Cell Dev Biol. 2016 May 6;4:40. doi: 10.3389/fcell.2016.00040. eCollection 2016.
3
Fluid shear stress promotes osteoblast proliferation via the Gαq-ERK5 signaling pathway.
J Pineal Res. 2025 Jul;77(4):e70062. doi: 10.1111/jpi.70062.
4
Effects of 0.01 mM strontium on human periodontal ligament stem cell osteogenic differentiation via the Wnt/-catenin signaling pathway.0.01 mM 锶通过Wnt/β-连环蛋白信号通路对人牙周膜干细胞成骨分化的影响
J Int Med Res. 2025 Feb;53(2):3000605251315024. doi: 10.1177/03000605251315024.
5
Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects.多功能水凝胶对成骨微环境的程序化改造以促进感染性骨缺损的修复
Adv Sci (Weinh). 2025 Mar;12(10):e2409683. doi: 10.1002/advs.202409683. Epub 2025 Jan 22.
6
Influence of melatonin supplementation on cancer-related fatigue: a meta-analysis of randomized controlled trials.补充褪黑素对癌症相关疲劳的影响：一项随机对照试验的荟萃分析。
Clin Transl Oncol. 2024 Dec 29. doi: 10.1007/s12094-024-03824-7.
7
Evaluation of osteoblast and osteoclast differentiation from stem cell: a systematic review of morphological assays and staining techniques.评价骨髓间充质干细胞向成骨细胞和破骨细胞分化：形态学检测和染色技术的系统评价。
PeerJ. 2024 Jul 25;12:e17790. doi: 10.7717/peerj.17790. eCollection 2024.
8
Investigating the Effects and Mechanisms of Combined Vitamin D and K Supplementation in Postmenopausal Women: An Up-to-Date Comprehensive Review of Clinical Studies.探讨维生素 D 和 K 联合补充对绝经后妇女的影响及其作用机制：临床研究的最新综合综述。
Nutrients. 2024 Jul 20;16(14):2356. doi: 10.3390/nu16142356.
9
Use of Local Melatonin with Xenogeneic Bone Graft to Treat Critical-Size Bone Defects in Rats with Osteoporosis: A Randomized Study.局部应用褪黑素联合异种骨移植治疗骨质疏松大鼠临界尺寸骨缺损：一项随机研究。
J Funct Biomater. 2024 May 13;15(5):124. doi: 10.3390/jfb15050124.
10
The Potential Use of Vitamin D3 and Phytochemicals for Their Anti-Ageing Effects.维生素 D3 和植物化学物质在抗衰老方面的潜在用途。
Int J Mol Sci. 2024 Feb 9;25(4):2125. doi: 10.3390/ijms25042125.
流体剪切应力通过Gαq-ERK5信号通路促进成骨细胞增殖。
Connect Tissue Res. 2016 Jul;57(4):299-306. doi: 10.1080/03008207.2016.1181063. Epub 2016 Apr 26.
4
Changes in Nonosteoporotic Bone Density and Subsequent Fractures in Women.女性非骨质疏松性骨密度变化及随后的骨折情况
South Med J. 2016 Feb;109(2):118-23. doi: 10.14423/SMJ.0000000000000410.
5
Epigenetic Control of Skeletal Development by the Histone Methyltransferase Ezh2.组蛋白甲基转移酶Ezh2对骨骼发育的表观遗传调控
J Biol Chem. 2015 Nov 13;290(46):27604-17. doi: 10.1074/jbc.M115.672345. Epub 2015 Sep 30.
6
Calcium intake and risk of fracture: systematic review.钙摄入量与骨折风险：系统评价
BMJ. 2015 Sep 29;351:h4580. doi: 10.1136/bmj.h4580.
7
Reduced fat mass and increased lean mass in response to 1 year of melatonin treatment in postmenopausal women: A randomized placebo-controlled trial.绝经后女性接受褪黑素治疗1年后脂肪量减少、瘦体重增加：一项随机安慰剂对照试验。
Clin Endocrinol (Oxf). 2016 Mar;84(3):342-7. doi: 10.1111/cen.12942. Epub 2015 Oct 8.
8
Melatonin improves bone mineral density at the femoral neck in postmenopausal women with osteopenia: a randomized controlled trial.褪黑素可改善绝经后骨质疏松症女性股骨颈骨密度：一项随机对照试验。
J Pineal Res. 2015 Sep;59(2):221-9. doi: 10.1111/jpi.12252. Epub 2015 Jun 24.
9
MEK5 suppresses osteoblastic differentiation.MEK5抑制成骨细胞分化。
Biochem Biophys Res Commun. 2015 Jul 31;463(3):241-7. doi: 10.1016/j.bbrc.2015.05.035. Epub 2015 May 19.
10
Vitamin D and bone mineral density changes in postmenopausal women treated with strontium ranelate.雷奈酸锶治疗绝经后妇女的维生素 D 和骨矿物质密度变化。
J Endocrinol Invest. 2015 Aug;38(8):859-63. doi: 10.1007/s40618-015-0299-2. Epub 2015 May 8.