• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过“过滤”方法合成环状寡脱氧核糖核苷酸。

Synthesis of cyclic oligodeoxyribonucleotides via the 'filtration' approach.

作者信息

Rao M V, Reese C B

机构信息

Department of Chemistry, King's College London, Strand, UK.

出版信息

Nucleic Acids Res. 1989 Oct 25;17(20):8221-39. doi: 10.1093/nar/17.20.8221.

DOI:10.1093/nar/17.20.8221
PMID:2813060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334960/
Abstract

The synthesis of cyclic di-, tri-, tetra-, penta- and hexa-thymidylic acids (16; n = 2, 3, 4, 5 and 6) and the cyclic hexadeoxyribonucleotide [d(CpCpTpApGpGp)], via the "filtration" approach, is reported. Some of the physical properties of the cyclic oligonucleotides are discussed, and their susceptibility to digestion in the presence of phosphorylytic enzymes has been studied.

摘要

报道了通过“过滤”方法合成环状二聚、三聚、四聚、五聚和六聚胸苷酸（16；n = 2、3、4、5和6）以及环状十六脱氧核糖核苷酸[d(CpCpTpApGpGp)]。讨论了环状寡核苷酸的一些物理性质，并研究了它们在磷酸解酶存在下的消化敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8be0/334960/959f72307e46/nar00137-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8be0/334960/959f72307e46/nar00137-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8be0/334960/959f72307e46/nar00137-0200-a.jpg

相似文献

1
Synthesis of cyclic oligodeoxyribonucleotides via the 'filtration' approach.通过“过滤”方法合成环状寡脱氧核糖核苷酸。
Nucleic Acids Res. 1989 Oct 25;17(20):8221-39. doi: 10.1093/nar/17.20.8221.
2
Synthesis and purification of thio-oligonucleotides.硫代寡核苷酸的合成与纯化。
Nucleic Acids Symp Ser. 1991(24):288.
3
Large scale automated synthesis of oligonucleotides.寡核苷酸的大规模自动化合成
Nucleic Acids Symp Ser. 1991(24):41-2.
4
Synthesis of cyclic oligonucleotides by a modified phosphotriester approach.通过改良的磷酸三酯法合成环状寡核苷酸。
Nucleic Acids Res. 1988 May 25;16(10):4607-20. doi: 10.1093/nar/16.10.4607.
5
Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.在聚酰胺载体上固相磷三酯法合成大型寡脱氧核糖核苷酸。
Nucleic Acids Res. 1980 Nov 25;8(22):5193-205. doi: 10.1093/nar/8.22.5193.
6
One pot solution synthesis of cyclic oligodeoxyribonucleotides.一锅法合成环状寡脱氧核糖核苷酸。
Nucleic Acids Res. 1990 May 11;18(9):2661-9. doi: 10.1093/nar/18.9.2661.
7
Functionalization of oligonucleotides by the incorporation of thio-specific reporter groups.通过掺入硫特异性报告基团实现寡核苷酸的功能化。
Methods Mol Biol. 1994;26:121-43. doi: 10.1007/978-1-59259-513-6_4.
8
Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.多核苷酸的固相合成。II. 用片段偶联磷酸三酯法合成聚胸腺嘧啶核苷酸
Nucleic Acids Res. 1980 Nov 25;8(22):5473-89. doi: 10.1093/nar/8.22.5473.
9
Functionalization of oligonucleotides with amino groups and attachment of amino specific reporter groups.用氨基对寡核苷酸进行功能化修饰以及连接氨基特异性报告基团。
Methods Mol Biol. 1994;26:93-120. doi: 10.1007/978-1-59259-513-6_3.
10
Application of 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite for in situ preparation of deoxyribonucleoside phosphoramidites and their use in polymer-supported synthesis of oligodeoxyribonucleotides.2-氰基乙基-N,N,N',N'-四异丙基亚磷酰胺在原位制备脱氧核糖核苷亚磷酰胺中的应用及其在聚合物支持的寡脱氧核苷酸合成中的用途。
Nucleic Acids Res. 1986 Sep 25;14(18):7391-403. doi: 10.1093/nar/14.18.7391.

引用本文的文献

1
Preorganization of DNA: Design Principles for Improving Nucleic Acid Recognition by Synthetic Oligonucleotides.DNA的预组织：通过合成寡核苷酸改善核酸识别的设计原则。
Chem Rev. 1997 Aug 5;97(5):1473-1488. doi: 10.1021/cr9603791.
2
Design and synthesis of RNA miniduplexes via a synthetic linker approach. 2. Generation of covalently closed, double-stranded cyclic HIV-1 TAR RNA analogs with high Tat-binding affinity.通过合成连接子方法设计与合成RNA小双链体。2. 生成具有高Tat结合亲和力的共价闭合双链环状HIV-1 TAR RNA类似物。
Nucleic Acids Res. 1993 Jun 11;21(11):2585-9. doi: 10.1093/nar/21.11.2585.
3
Oligonucleotide circularization by template-directed chemical ligation.

本文引用的文献

1
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.
2
Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.一些与肟离子促进寡核苷酸芳基酯解封闭相关的观察结果。
Nucleic Acids Res. 1981 Sep 25;9(18):4611-26. doi: 10.1093/nar/9.18.4611.
3
Synthesis of cyclic oligonucleotides by a modified phosphotriester approach.
通过模板导向化学连接实现寡核苷酸环化
Nucleic Acids Res. 1993 Nov 25;21(23):5403-7. doi: 10.1093/nar/21.23.5403.
4
Circulation of oligonucleotides by disulfide bridge formation.通过二硫键形成实现寡核苷酸的循环。
Nucleic Acids Res. 1995 Jun 11;23(11):2025-9. doi: 10.1093/nar/23.11.2025.
5
One pot solution synthesis of cyclic oligodeoxyribonucleotides.一锅法合成环状寡脱氧核糖核苷酸。
Nucleic Acids Res. 1990 May 11;18(9):2661-9. doi: 10.1093/nar/18.9.2661.
通过改良的磷酸三酯法合成环状寡核苷酸。
Nucleic Acids Res. 1988 May 25;16(10):4607-20. doi: 10.1093/nar/16.10.4607.
4
Prevention of chain cleavage in the chemical synthesis of 2'-silylated oligoribonucleotides.2'-硅烷化寡核糖核苷酸化学合成中链裂解的预防
Nucleic Acids Res. 1989 May 11;17(9):3501-17. doi: 10.1093/nar/17.9.3501.