• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在聚酰胺载体上固相磷三酯法合成大型寡脱氧核糖核苷酸。

Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.

作者信息

Markham A F, Edge M D, Atkinson T C, Greene A R, Heathcliffe G R, Newton C R, Scanlon D

出版信息

Nucleic Acids Res. 1980 Nov 25;8(22):5193-205. doi: 10.1093/nar/8.22.5193.

DOI:10.1093/nar/8.22.5193
PMID:7465412
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324294/
Abstract

Phosphotriester solid phase methodology on a polyamide support [(1980) Nucleic Acids Research, 8, 1081-1096] has been extended for the rapid synthesis of the tetradecanucleotide, d(AGTTGTTTGTAGTT), the octadecanucleotide, d(GTGGGTTTGGGGCAGGTC), and the heneicosanucleotide, d(GTGCTCTTATCCTCTTGGCTC). Thus, oligodeoxyribonucleotides comparable in size to those obtained by solution synthesis are readily accessible using solid phase techniques. An approach to the purification of the synthetic octadecanucleotide without recourse to high performance liquid chromatography is described.

摘要

聚酰胺载体上的磷酸三酯固相合成方法[(1980年，《核酸研究》，第8卷，1081 - 1096页)]已得到扩展，用于快速合成十四聚体d(AGTTGTTTGTAGTT)、十八聚体d(GTGGGTTTGGGGCAGGTC)和二十一聚体d(GTGCTCTTATCCTCTTGGCTC)。因此，使用固相技术可以很容易地获得与溶液合成法得到的大小相当的寡脱氧核糖核苷酸。本文描述了一种无需借助高效液相色谱法来纯化合成十八聚体的方法。

相似文献

1
Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.在聚酰胺载体上固相磷三酯法合成大型寡脱氧核糖核苷酸。
Nucleic Acids Res. 1980 Nov 25;8(22):5193-205. doi: 10.1093/nar/8.22.5193.
2
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.寡脱氧核糖核苷酸的快速合成。VII. 在硅藻土-聚酰胺载体上通过连续流动磷酸三酯法固相合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243.
3
Rapid synthesis of oligodeoxyribonucleotides. V. Further studies in solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡聚脱氧核糖核苷酸的快速合成。V. 通过磷酸三酯中间体进行寡聚脱氧核糖核苷酸固相合成的进一步研究。
Nucleic Acids Symp Ser. 1980(7):243-57.
4
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.
5
Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method.多核苷酸的固相合成。VIII. 用磷酸三酯固相法合成混合寡脱氧核糖核苷酸。
Nucleic Acids Res. 1983 Jan 25;11(2):477-88. doi: 10.1093/nar/11.2.477.
6
Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。
Nucleic Acids Res. 1981 Apr 10;9(7):1691-706. doi: 10.1093/nar/9.7.1691.
7
A rapid deprotection procedure for phosphotriester DNA synthesis.一种用于磷酸三酯DNA合成的快速脱保护程序。
Nucleic Acids Res. 1984 Sep 11;12(17):6853-9. doi: 10.1093/nar/12.17.6853.
8
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.通过磷酸三酯中间体合成寡核苷酸的新有效方法。
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675.
9
Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.硅胶：用于寡核苷酸固相磷酸三酯合成的一种改进载体。
Nucleic Acids Res. 1982 Nov 25;10(22):7439-48. doi: 10.1093/nar/10.22.7439.
10
Continuous-flow phosphotriester method for solid-phase synthesis of oligodeoxyribonucleotides.用于寡脱氧核糖核苷酸固相合成的连续流动磷酸三酯法。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:393-401. doi: 10.1101/sqb.1983.047.01.046.

引用本文的文献

1
Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。
Nucleic Acids Res. 1981 Apr 10;9(7):1691-706. doi: 10.1093/nar/9.7.1691.
2
Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.脱氧寡核苷酸固相磷三酯合成法的改进
Nucleic Acids Res. 1982 Sep 25;10(18):5605-20. doi: 10.1093/nar/10.18.5605.
3
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

本文引用的文献

1
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.
2
Synthesis of oligonucleotides on cellulose by a phosphotriester method.通过磷酸三酯法在纤维素上合成寡核苷酸。
Nucleic Acids Res. 1980 May 24;8(10):2331-48. doi: 10.1093/nar/8.10.2331.
3
Sequencing end-labeled DNA with base-specific chemical cleavages.
多核苷酸的固相合成。VI. 关于用于固相载体的聚苯乙烯共聚物的进一步研究。
Nucleic Acids Res. 1982 Mar 11;10(5):1755-69. doi: 10.1093/nar/10.5.1755.
4
Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.人类补体蛋白B因子（一种III类主要组织相容性复合体基因产物）的cDNA克隆的分离
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5661-5. doi: 10.1073/pnas.79.18.5661.
5
Direct detection of the common Mediterranean beta-thalassemia gene with synthetic DNA probes. An alternative approach for prenatal diagnosis.用合成DNA探针直接检测常见的地中海β-地中海贫血基因。产前诊断的另一种方法。
J Clin Invest. 1983 Mar;71(3):775-9. doi: 10.1172/jci110826.
6
Recent developments in the chemical synthesis of polynucleotides.多核苷酸化学合成的最新进展。
Nucleic Acids Res. 1982 Nov 11;10(21):6553-70. doi: 10.1093/nar/10.21.6553.
7
Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.人α-2干扰素基因的化学合成及其在大肠杆菌中的表达。
Nucleic Acids Res. 1983 Sep 24;11(18):6419-35. doi: 10.1093/nar/11.18.6419.
8
Synthesis of oligodeoxyribonucleotides on silica gel support.硅胶载体上寡脱氧核糖核苷酸的合成。
Nucleic Acids Res. 1981 Jun 25;9(12):2807-17. doi: 10.1093/nar/9.12.2807.
9
The isolation of cDNA clones for human apolipoprotein E and the detection of apoE RNA in hepatic and extra-hepatic tissues.人载脂蛋白E的cDNA克隆分离及肝组织和肝外组织中载脂蛋白E RNA的检测。
EMBO J. 1983;2(12):2369-73. doi: 10.1002/j.1460-2075.1983.tb01748.x.
10
Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase.人X染色体编码的磷酸甘油酸激酶全长cDNA克隆的分离与DNA序列分析
Proc Natl Acad Sci U S A. 1983 Jan;80(2):472-6. doi: 10.1073/pnas.80.2.472.
通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
4
Letter: Polyamide supports for polypeptide synthesis.信函：用于多肽合成的聚酰胺载体
J Am Chem Soc. 1975 Oct 29;97(22):6584-5. doi: 10.1021/ja00855a053.
5
High-pressure liquid chromatography in polynucleotide synthesis.多核苷酸合成中的高压液相色谱法。
Biochemistry. 1978 Apr 4;17(7):1257-67. doi: 10.1021/bi00600a020.
6
Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide.寡脱氧核糖核苷酸的快速合成。II. 两种九核苷酸和一种八核苷酸的机器辅助固相合成
Nucleic Acids Res. 1977 Dec;4(12):4391-410. doi: 10.1093/nar/4.12.4391.
7
Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support.在接枝聚合物载体上快速合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1979;6(6):2041-56. doi: 10.1093/nar/6.6.2041.
8
Synthesis and cloning of operator DNA of the lac operon of E. coli.大肠杆菌乳糖操纵子操纵基因DNA的合成与克隆
Can J Biochem. 1977 Nov;55(11):1125-33. doi: 10.1139/o77-168.
9
Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA.人胰岛素基因的合成。第二部分。改良磷酸三酯法的进一步改进以及构成人胰岛素B链和微型cDNA的17个脱氧核糖寡核苷酸片段的合成。
Nucleic Acids Res. 1979 Dec 20;7(8):2199-212. doi: 10.1093/nar/7.8.2199.
10
Protected deoxyribonucleoside-3' aryl phosphodiesters as key intermediates in polynucleotide synthesis. Construction of an icosanucleotide analogous to the sequence at the ends of Rous sarcoma virus 35S RNA.作为多核苷酸合成关键中间体的受保护脱氧核糖核苷-3'-芳基磷酸二酯。构建与劳氏肉瘤病毒35S RNA末端序列类似的二十核苷酸。
Nucleic Acids Res. 1979 Apr;6(4):1557-70. doi: 10.1093/nar/6.4.1557.