寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.
作者信息
Gait M J, Singh M, Sheppard R C, Edge M D, Greene A R, Heathcliffe G R, Atkinson T C, Newton C R, Markham A F
出版信息
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.
Abstract
A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields.
摘要
一种在聚酰胺载体上的磷酸三酯固相法已被用于制备长度达12个单元的寡脱氧核糖核苷酸。与固相磷酸二酯合成相比，新方法更快、更灵活，且总产率提高了10至60倍。
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OLIGONUCLEOTIDE SYNTHESIS ON A POLYMER SUPPORT.聚合物载体上的寡核苷酸合成
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A new polymer-support method for the synthesis of ribooligonucleotide.一种用于合成核糖寡核苷酸的新型聚合物支持方法。
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Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.用于检测肽固相合成中游离末端氨基的颜色测试。
Anal Biochem. 1970 Apr;34(2):595-8. doi: 10.1016/0003-2697(70)90146-6.
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A rapid and convenient synthesis of poly-thymidylic acid by the modified triester approach.通过改进的三酯法快速便捷地合成聚胸腺嘧啶核苷酸。
Nucleic Acids Res. 1977 Aug;4(8):2757-65. doi: 10.1093/nar/4.8.2757.
6
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High-pressure liquid chromatography in polynucleotide synthesis.多核苷酸合成中的高压液相色谱法。
Biochemistry. 1978 Apr 4;17(7):1257-67. doi: 10.1021/bi00600a020.
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Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide.寡脱氧核糖核苷酸的快速合成。II. 两种九核苷酸和一种八核苷酸的机器辅助固相合成
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Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribooligonucleotides fragments constituting human insulin A DNA.人胰岛素基因的合成。第一部分。改良三酯法中反相色谱的发展。其在快速高效合成构成人胰岛素A DNA的八个脱氧核糖寡核苷酸片段中的应用。
Nucleic Acids Res. 1979 Apr;6(4):1371-85. doi: 10.1093/nar/6.4.1371.
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Rapid synthesis of oligodeoxyribonucleotides. III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T).
Nucleic Acids Res. 1979 Apr;6(4):1259-68. doi: 10.1093/nar/6.4.1259.
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