Kalitnik A A, Karetin Y A, Kravchenko A O, Khasina E I, Yermak I M
School of Biomedicine, School of Natural Sciences Far Eastern Federal University, 8 Sukhanova Street, Vladivostok, 690950.
A. V. Zhirmunsky Institute of Marine Biology, FEB RAS, 17 Palchevskogo street, Vladivostok, 690022.
J Biomed Mater Res A. 2017 May;105(5):1549-1557. doi: 10.1002/jbm.a.36015. Epub 2017 Mar 23.
The in vivo effect of κ/β-carrageenan isolated from the red alga Tichocarpus crinitus on cytokine synthesis and cellular activity of murine peritoneal macrophages and also the protective effect of polysaccharides in LPS-induced endotoxemia in mice was studied. It was established that κ/β-carrageenan given orally at a dose of 100 mg/kg stimulates the induction of anti-inflammatory cytokines (IL-10) in mouse blood cells by more than 2.5-fold compared with control, with no effect on pro-inflammatory cytokine (TNF-α) production. Pretreating mice with carrageenan once a day before injecting LPS increased the levels of IL-10 by 2.5-fold and reduced TNF-α production by 2-fold compared with control. So, κ/β-carrageenan alone and in combination with LPS enhanced the cellular activity and mobility of peritoneal macrophages by increasing cell adhesion and migration compared with control. LPS activated cells intensively, sometimes resulting in their destruction by necrosis; carrageenan pretreatment reduced the excessive inflammatory cell activation caused by LPS. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1549-1557, 2017.
研究了从红藻繁枝蜈蚣藻中分离出的κ/β-角叉菜胶对小鼠腹腔巨噬细胞细胞因子合成和细胞活性的体内作用，以及多糖对小鼠脂多糖诱导的内毒素血症的保护作用。结果表明，以100 mg/kg的剂量口服κ/β-角叉菜胶，与对照组相比，可使小鼠血细胞中抗炎细胞因子（IL-10）的诱导增加2.5倍以上，而对促炎细胞因子（TNF-α）的产生没有影响。在注射脂多糖前每天用角叉菜胶预处理小鼠一次，与对照组相比，IL-10水平增加2.5倍，TNF-α产生减少2倍。因此，与对照组相比，单独的κ/β-角叉菜胶以及与脂多糖联合使用，均可通过增加细胞黏附和迁移来增强腹腔巨噬细胞的细胞活性和迁移能力。脂多糖强烈激活细胞，有时会导致细胞坏死；角叉菜胶预处理可减少脂多糖引起的过度炎症细胞激活。© 2017威利期刊公司。《生物医学材料研究杂志》A部分：105A：1549 - 1557，2017年。
