G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
Mar Drugs. 2020 May 10;18(5):248. doi: 10.3390/md18050248.
The inhibitory effects of carrageenans (CRGs) on lipopolysaccharide (LPS) induced inflammation in a mouse model of endotoxemia and in complex therapy of patients with enteric infections of Salmonella etiology were studied. The atomic force microscopy (AFM) examination of LPS and its mixture with CRGs showed that the LPS morphology is significantly changed under the action of κ- and κ/β-CRGs. CRGs were able to increase the synthesis of anti-inflammatory interleukin 10 (IL-10) in vitro, and, at low concentrations, their activity in the mixture with LPS was higher. The protective effect of CRGs against LPS was studied in vivo by monitoring the biochemical and pathomorphological parameters. The κ- and κ/β-CRGs and food supplement "Carrageenan-FE" increased the nonspecific resistance of mice to LPS at the expense of the inhibition of processes of thymus involution, adrenals hypertrophy, thyroid atrophy, hypercorticoidism, glycogenolysis, and lactate acidosis. The estimation of the therapeutic action of food supplement Carrageenan-FE in complex therapy of patients with enteric infections of Salmonella etiology is given. Carrageenan-FE restores the system of hemostasis and corrects some biochemical indicators and parameters in the immune systems of patients. These results allow us to hope for the practical application of CRGs for lowering the endotoxemia level in patients under the development of the infectious process caused by Gram-negative bacteria.
研究了卡拉胶（CRGs）对脂多糖（LPS）诱导的内毒素血症小鼠模型炎症的抑制作用，以及在沙门氏菌病因肠道感染的综合治疗中的作用。原子力显微镜（AFM）检查 LPS 及其与 CRGs 的混合物表明，LPS 的形态在 κ-和 κ/β-CRG 的作用下发生了显著变化。CRGs 能够在体外增加抗炎白细胞介素 10（IL-10）的合成，并且在与 LPS 的混合物中，其低浓度下的活性更高。通过监测生化和病理形态学参数，在体内研究了 CRGs 对 LPS 的保护作用。κ-和 κ/β-CRG 和食品补充剂“卡拉胶-FE”通过抑制胸腺萎缩、肾上腺肥大、甲状腺萎缩、皮质醇过多、糖原分解和乳酸酸中毒等过程，提高了 LPS 对小鼠的非特异性抵抗力。给出了食品补充剂卡拉胶-FE 在沙门氏菌病因肠道感染的综合治疗中的治疗作用的估计。卡拉胶-FE 恢复了止血系统，并纠正了患者免疫系统中的一些生化指标和参数。这些结果使我们有希望在革兰氏阴性菌引起的感染过程中，将 CRGs 实际应用于降低患者的内毒素血症水平。
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