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长链非编码RNA介导的春化作用引发的基因内染色质环形成

Vernalization-Triggered Intragenic Chromatin Loop Formation by Long Noncoding RNAs.

作者信息

Kim Dong-Hwan, Sung Sibum

机构信息

Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2506 Speedway, Austin, TX 78712, USA.

Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2506 Speedway, Austin, TX 78712, USA.

出版信息

Dev Cell. 2017 Feb 6;40(3):302-312.e4. doi: 10.1016/j.devcel.2016.12.021. Epub 2017 Jan 26.

DOI:10.1016/j.devcel.2016.12.021
PMID:28132848
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5303624/
Abstract

Long noncoding RNAs (lncRNAs) affect gene regulation through structural and regulatory interactions with associated proteins. The Polycomb complex often binds to lncRNAs in eukaryotes, and an lncRNA, COLDAIR, associates with Polycomb to mediate silencing of the floral repressor FLOWERING LOCUS C (FLC) during the process of vernalization in Arabidopsis. Here, we identified an additional Polycomb-binding lncRNA, COLDWRAP. COLDWRAP is derived from the repressed promoter of FLC and is necessary for the establishment of the stable repressed state of FLC by vernalization. Both COLDAIR and COLDWRAP are required to form a repressive intragenic chromatin loop at the FLC locus by vernalization. Our results indicate that vernalization-mediated Polycomb silencing is coordinated by lncRNAs in a cooperative manner to form a stable repressive chromatin structure.

摘要

长链非编码RNA（lncRNAs）通过与相关蛋白质的结构和调控相互作用来影响基因调控。在真核生物中，多梳蛋白复合体通常与lncRNAs结合，一种名为COLDAIR的lncRNA在拟南芥春化过程中与多梳蛋白结合，介导 floral repressor FLOWERING LOCUS C（FLC）的沉默。在此，我们鉴定出另一种与多梳蛋白结合的lncRNA，即COLDWRAP。COLDWRAP源自FLC的受抑制启动子，是春化作用使FLC建立稳定抑制状态所必需的。COLDAIR和COLDWRAP都是春化作用在FLC基因座形成抑制性基因内染色质环所必需的。我们的结果表明，春化介导的多梳蛋白沉默由lncRNAs以协同方式协调，以形成稳定的抑制性染色质结构。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/b09292412231/nihms847002f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/f2819e0047a3/nihms847002f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/47c8bc9b7cda/nihms847002f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/3923374a7baf/nihms847002f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/1ab24304627c/nihms847002f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/353c2eff5fc6/nihms847002f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/b09292412231/nihms847002f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/f2819e0047a3/nihms847002f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/47c8bc9b7cda/nihms847002f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/3923374a7baf/nihms847002f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/1ab24304627c/nihms847002f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/353c2eff5fc6/nihms847002f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bfb/5303624/b09292412231/nihms847002f6.jpg

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