Fischbarg J, Kuang K Y, Hirsch J, Lecuona S, Rogozinski L, Silverstein S C, Loike J
Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Proc Natl Acad Sci U S A. 1989 Nov;86(21):8397-401. doi: 10.1073/pnas.86.21.8397.
Water transport across plasma membranes is a universal property of cells, but the route of such transport is unclear. In this study, volume changes of cells of the J774 murine macrophage-like cell line were monitored by recording the intensity of light scattered by the cells. We investigated the effects of several inhibitors of glucose transport on cell membrane osmotic water permeability as calculated from the rates of cell volume change. Cytochalasin B (2.5 micrograms/ml), phloretin (20 microM), and tomatine (3 microM) reversibly blocked glucose uptake into these cells. All three inhibitors reversibly decreased the osmotic water permeability of J774 cells from 89.6 +/- 3.2 to 27.2 +/- 1.4 microns/sec. We conclude that a major component of the osmotic water flow across the plasma membranes of these cells is accounted for by water traversing their glucose transporters.
水通过质膜的运输是细胞的普遍特性，但这种运输的途径尚不清楚。在本研究中，通过记录细胞散射光的强度来监测J774鼠巨噬细胞样细胞系细胞的体积变化。我们根据细胞体积变化率计算了几种葡萄糖转运抑制剂对细胞膜渗透水通透性的影响。细胞松弛素B（2.5微克/毫升）、根皮素（20微摩尔）和番茄碱（3微摩尔）可逆转地阻断这些细胞对葡萄糖的摄取。所有这三种抑制剂均可逆转地将J774细胞的渗透水通透性从89.6±3.2微米/秒降低至27.2±1.4微米/秒。我们得出结论，这些细胞质膜上渗透水流的一个主要成分是水通过其葡萄糖转运体。
