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肿瘤坏死因子可诱导人宫颈癌细胞中一种28 kDa的mRNA帽结合蛋白发生磷酸化。

Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells.

作者信息

Marino M W, Pfeffer L M, Guidon P T, Donner D B

机构信息

Memorial Sloan-Kettering Cancer Center, New York, NY 10021.

出版信息

Proc Natl Acad Sci U S A. 1989 Nov;86(21):8417-21. doi: 10.1073/pnas.86.21.8417.

DOI:10.1073/pnas.86.21.8417
PMID:2813400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC298293/
Abstract

Tumor necrosis factor alpha (TNF-alpha) stimulated the phosphorylation of a 28-kDa protein (p28) in the ME-180 line of human cervical carcinoma cells. The effect of TNF-alpha on the phosphorylation state of p28 was rapid (4-fold increase within 15 min) and persistent, remaining above the basal level for at least 2 hr. The specific binding of 125I-labeled TNF-alpha to cell-surface binding sites, the stimulation of p28 phosphorylation by TNF-alpha, and the inhibition of cell proliferation by TNF-alpha occurred with nearly identical dose-response relationships. Two-dimensional SDS/PAGE resolved p28 into two isoforms having pI values of 6.2 and 6.1. A phosphorylated cap-binding protein was substantially enriched from lysates of control or TNF-alpha-treated ME-180 cells by affinity chromatography with 7-methylguanosine 5'-triphosphate-Sepharose. The phosphoprotein recovered from this procedure was the substrate for TNF-alpha-promoted phosphorylation, p28. Thus, TNF-alpha stimulates the phosphorylation of this mRNA cap-binding protein, which may be involved in the transduction of TNF-alpha-receptor binding into cellular responses.

摘要

肿瘤坏死因子α（TNF-α）刺激人子宫颈癌细胞ME-180系中一种28 kDa蛋白（p28）的磷酸化。TNF-α对p28磷酸化状态的影响迅速（15分钟内增加4倍）且持续，至少2小时内维持在基础水平之上。125I标记的TNF-α与细胞表面结合位点的特异性结合、TNF-α对p28磷酸化的刺激以及TNF-α对细胞增殖的抑制，其剂量反应关系几乎相同。二维SDS/PAGE将p28解析为两种等电点分别为6.2和6.1的亚型。通过用7-甲基鸟苷5'-三磷酸-琼脂糖进行亲和层析，从对照或TNF-α处理的ME-180细胞裂解物中大量富集了一种磷酸化的帽结合蛋白。从该过程中回收的磷蛋白是TNF-α促进磷酸化的底物p28。因此，TNF-α刺激这种mRNA帽结合蛋白的磷酸化，这可能参与了TNF-α受体结合向细胞反应的转导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/e25d8389b15b/pnas00288-0255-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/36ad998d2a7d/pnas00288-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/06b3d5b52600/pnas00288-0253-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/4bba091c9b54/pnas00288-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/ea8769bdc8c7/pnas00288-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/e25d8389b15b/pnas00288-0255-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/36ad998d2a7d/pnas00288-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/06b3d5b52600/pnas00288-0253-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/4bba091c9b54/pnas00288-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/ea8769bdc8c7/pnas00288-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c7e/298293/e25d8389b15b/pnas00288-0255-b.jpg

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本文引用的文献

1
Purification of the messenger RNA cap-binding protein using a new affinity medium.使用新型亲和介质纯化信使核糖核酸帽结合蛋白。
Biochemistry. 1984 Jan 17;23(2):177-81. doi: 10.1021/bi00297a001.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro.重组人肿瘤坏死因子-α：对体外正常细胞和转化细胞增殖的影响
肿瘤坏死因子诱导的细胞毒性伴随着ME-180人宫颈癌细胞内的促有丝分裂信号。
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):185-90. doi: 10.1042/bj2900185.
4
Growth arrest of the breast cancer cell line, T47D, by TNF alpha; cell cycle specificity and signal transduction.肿瘤坏死因子α对乳腺癌细胞系T47D的生长抑制作用；细胞周期特异性及信号转导
Br J Cancer. 1993 Feb;67(2):290-6. doi: 10.1038/bjc.1993.55.
5
Modification of eukaryotic initiation factor 4F during infection by influenza virus.流感病毒感染期间真核起始因子4F的修饰。
J Virol. 1993 Jun;67(6):3027-35. doi: 10.1128/JVI.67.6.3027-3035.1993.
6
A late adenovirus factor induces eIF-4E dephosphorylation and inhibition of cell protein synthesis.一种晚期腺病毒因子可诱导真核起始因子4E（eIF-4E）去磷酸化并抑制细胞蛋白质合成。
J Virol. 1994 Nov;68(11):7040-50. doi: 10.1128/JVI.68.11.7040-7050.1994.
7
Phosphorylation of eukaryotic translation initiation factor 4E is increased in Src-transformed cell lines.真核生物翻译起始因子4E的磷酸化在Src转化的细胞系中增加。
Mol Cell Biol. 1991 May;11(5):2896-900. doi: 10.1128/mcb.11.5.2896-2900.1991.
8
Characterization of a ceramide-activated protein kinase: stimulation by tumor necrosis factor alpha.一种神经酰胺激活蛋白激酶的特性：受肿瘤坏死因子α刺激
Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10009-13. doi: 10.1073/pnas.88.22.10009.
9
Tumour necrosis factor-alpha induces translocation of protein kinase C in tumour necrosis factor-sensitive cell lines.肿瘤坏死因子-α可诱导肿瘤坏死因子敏感细胞系中蛋白激酶C的易位。
Immunology. 1991 Aug;73(4):457-9.
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Tumor necrosis factor induces rapid production of 1'2'diacylglycerol by a phosphatidylcholine-specific phospholipase C.肿瘤坏死因子通过磷脂酰胆碱特异性磷脂酶C诱导1,2 - 二酰甘油的快速产生。
J Exp Med. 1991 Nov 1;174(5):975-88. doi: 10.1084/jem.174.5.975.
Science. 1985 Nov 22;230(4728):943-5. doi: 10.1126/science.3933111.
4
A macrophage factor inhibits adipocyte gene expression: an in vitro model of cachexia.一种巨噬细胞因子抑制脂肪细胞基因表达：恶病质的体外模型。
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5
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J Biol Chem. 1987 Jan 5;262(1):380-8.
6
Effect of growth hormone on protein phosphorylation in isolated rat hepatocytes.生长激素对离体大鼠肝细胞中蛋白质磷酸化的影响。
Biochemistry. 1987 Feb 10;26(3):715-21. doi: 10.1021/bi00377a009.
7
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J Exp Med. 1986 Mar 1;163(3):632-43. doi: 10.1084/jem.163.3.632.
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Nature. 1986;323(6083):79-82. doi: 10.1038/323079a0.
9
Tumor necrosis factor stimulates interleukin-1 and prostaglandin E2 production in resting macrophages.肿瘤坏死因子刺激静息巨噬细胞产生白细胞介素-1和前列腺素E2。
Biochem Biophys Res Commun. 1986 Apr 14;136(1):94-101. doi: 10.1016/0006-291x(86)90881-8.
10
Interleukin 1 and tumour necrosis factor increase phosphorylation of fibroblast proteins.白细胞介素1和肿瘤坏死因子可增加成纤维细胞蛋白的磷酸化。
FEBS Lett. 1988 Dec 5;241(1-2):6-10. doi: 10.1016/0014-5793(88)81019-6.