结核分枝杆菌中的一些同义突变和非同义突变会导致使用海因基因分型MTBDR检测方法时出现系统性的氟喹诺酮耐药性假阳性结果。
Some Synonymous and Nonsynonymous Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDR Assays.
作者信息
Ajileye Adebisi, Alvarez Nataly, Merker Matthias, Walker Timothy M, Akter Suriya, Brown Kerstin, Moradigaravand Danesh, Schön Thomas, Andres Sönke, Schleusener Viola, Omar Shaheed V, Coll Francesc, Huang Hairong, Diel Roland, Ismail Nazir, Parkhill Julian, de Jong Bouke C, Peto Tim E A, Crook Derrick W, Niemann Stefan, Robledo Jaime, Smith E Grace, Peacock Sharon J, Köser Claudio U
机构信息
Public Health England West Midlands Public Health Laboratory, Heartlands Hospital, Birmingham, United Kingdom.
Bacteriology and Mycobacteria Unit, Corporación Para Investigaciones Biológicas, Medellín, Colombia.
出版信息
Antimicrob Agents Chemother. 2017 Mar 24;61(4). doi: 10.1128/AAC.02169-16. Print 2017 Apr.
Abstract
In this study, using the Hain GenoType MTBDR assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in in result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.
摘要
在本研究中，我们使用Hain GenoType MTBDR检测法（版本1和版本2）发现，katG基因中的一些非同义突变和同义突变通过阻止野生型探针的结合，导致对氟喹诺酮类药物出现系统性的假耐药结果。此外，此类突变可阻止为鉴定特定耐药突变而设计的突变探针的结合。尽管这些突变在全球范围内可能很少见，但在某些地区，约7%的耐多药结核菌株中会出现这些突变。
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