Raut Jayant Shankar, Bansode Bhagyashree Shridhar, Jadhav Ashwini Khanderao, Karuppayil Sankunny Mohan
University Institute of Pharmaceutical Sciences (UIPS), UGC-Centre of Advanced Study, Panjab University, Chandigarh-160014, India.
School of Life Sciences (DST-FIST & UGC-SAP sponsored), SRTM University, Nanded, 431606 (MH), India.
J Microbiol Biotechnol. 2017 Apr 28;27(4):685-693. doi: 10.4014/jmb.1607.07072.
Candidiasis involving the biofilms of is a threat to immunocompromised patients. biofilms are intrinsically resistant to the antifungal drugs and hence novel treatment strategies are desired. The study intended to evaluate the anti- activity of allyl isothiocyanate (AITC) alone and with fluconazole (FLC), particularly against the biofilms. Results revealed the concentration-dependent activity of AITC against the planktonic growth and virulence factors of . Significant ( <0.05) inhibition of the biofilms was evident at < or =1 mg/ml concentrations of AITC. Notably, a combination of 0.004 mg/ml of FLC and 0.125 mg/ml of AITC prevented the biofilm formation. Similarly, the preformed biofilms were significantly ( <0.05) inhibited by the AITC-FLC combination. The fractional inhibitory concentration indices ranging from 0.132 to 0.312 indicated the synergistic activity of AITC and FLC against the biofilm formation and the preformed biofilms. No hemolytic activity at the biofilm inhibitory concentrations of AITC and the AITC-FLC combination suggested the absence of cytotoxic effects. The recognizable synergy between AITC and FLC offers a potential therapeutic strategy against biofilm-associated infections.
念珠菌生物膜感染对免疫功能低下的患者构成威胁。生物膜对抗真菌药物具有内在抗性，因此需要新的治疗策略。该研究旨在评估异硫氰酸烯丙酯（AITC）单独以及与氟康唑（FLC）联合使用时的抗念珠菌活性，特别是针对生物膜的活性。结果显示AITC对念珠菌的浮游生长和毒力因子具有浓度依赖性活性。在AITC浓度≤1mg/ml时，对生物膜有显著（P<0.05）抑制作用。值得注意的是，0.004mg/ml的FLC与0.125mg/ml的AITC联合使用可防止生物膜形成。同样，AITC与FLC的联合使用对预先形成的生物膜也有显著（P<0.05）抑制作用。分级抑制浓度指数在0.132至0.312之间，表明AITC和FLC对生物膜形成及预先形成的生物膜具有协同活性。在AITC及AITC与FLC联合使用的生物膜抑制浓度下均无溶血活性，表明无细胞毒性作用。AITC与FLC之间明显的协同作用为生物膜相关念珠菌感染提供了一种潜在的治疗策略。
