Horie Yoshifumi, Yamagishi Takahiro, Koshio Masaaki, Iguchi Taisen, Tatarazako Norihisa
Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Ibaraki, Japan.
Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.
J Appl Toxicol. 2017 Jul;37(7):836-841. doi: 10.1002/jat.3431. Epub 2017 Jan 31.
Environmental risk assessments show increased attention to the sublethal effects of chemicals on aquatic organisms. The Organization for Economic Cooperation and Development (OECD) established the "Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages" (OECD test 212) to predict lethal effects. It is still unclear, however, whether this test can predict sublethal effects. Although their sublethal effects are still unknown, chlorinated anilines are widely used in various fields. The purpose of this study, therefore, is to investigate sublethal effects of chlorinated anilines using OECD test 212 with zebrafish, and to examine the correlation of several sublethal effects between embryo and larval stages. Embryos were exposed to aniline and nine chlorinated anilines until 8 days post-fertilization. A delayed lethal effect was observed from three of the 10 anilines tested. In the control group, the swim bladder inflated after hatching, but there was no swim-bladder inflation after exposure to the chlorinated anilines. Fertilized eggs exposed to lower concentrations of test chemicals showed effects during embryogenesis that did not affect mortality rates, such as changes in body curvature and edema. Our results show that chlorinated anilines induce not only lethal effects but also a variety of sublethal effects. Moreover, a detailed estimate of these effects requires study during both embryonic and larval stages. OECD test 212 may therefore prove useful as a method for screening chemicals for lethal and sublethal effects. Copyright © 2017 John Wiley & Sons, Ltd.
环境风险评估显示，人们越来越关注化学物质对水生生物的亚致死效应。经济合作与发展组织（OECD）制定了“鱼类胚胎和仔鱼期短期毒性试验”（OECD试验212）来预测致死效应。然而，该试验能否预测亚致死效应仍不清楚。尽管氯化苯胺的亚致死效应尚不清楚，但它们在各个领域都有广泛应用。因此，本研究的目的是使用斑马鱼通过OECD试验212来研究氯化苯胺的亚致死效应，并检验胚胎期和幼体期几种亚致死效应之间的相关性。将胚胎暴露于苯胺和九种氯化苯胺中，直至受精后8天。在所测试的10种苯胺中有3种观察到延迟致死效应。在对照组中，孵化后鱼鳔充气，但暴露于氯化苯胺后鱼鳔未充气。暴露于较低浓度试验化学品的受精卵在胚胎发育过程中出现了不影响死亡率的效应，如身体弯曲和水肿的变化。我们的结果表明，氯化苯胺不仅会导致致死效应，还会引发多种亚致死效应。此外，对这些效应的详细评估需要在胚胎期和幼体期进行研究。因此，OECD试验212可能被证明是一种筛选具有致死和亚致死效应化学品的有用方法。版权所有© 2017约翰·威利父子有限公司。
