Jankovsky Jennie M, Brand Mabre, Gerhold Richard W
1 Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, Tennessee 37996, USA.
J Wildl Dis. 2017 Apr;53(2):368-371. doi: 10.7589/2016-06-132. Epub 2017 Jan 31.
We diagnosed renal coccidiosis in two of five Great-horned Owls ( Bubo virginianus ) examined in eastern Tennessee, US, 2007-13. Histopathologic examination of the kidneys revealed multifocal mild-to-moderate dilation and epithelial hyperplasia of collecting ducts. Renal collecting duct epithelial cells contained intracytoplasmic microgametocytes, macrogametocytes, and sporulating and sporulated oocysts. Renal coccidiosis in affected birds did not result in significant inflammation. Sequence analysis of the amplified partial 18S short subunit ribosomal RNA coding region from examination of formalin fixed tissue by using PCR disclosed a 93% identity to Eimeria reichenowi in GenBank, suggesting a novel Eimeria sp.
2007年至2013年期间，在美国田纳西州东部检查的五只大角鸮（Bubo virginianus）中，我们诊断出两例肾球虫病。对肾脏进行组织病理学检查发现，集合管有多处轻度至中度扩张和上皮增生。肾集合管上皮细胞内含有胞质内小配子体、大配子体以及正在孢子化和已孢子化的卵囊。受感染鸟类的肾球虫病并未导致明显炎症。通过聚合酶链反应（PCR）对福尔马林固定组织进行检查，对扩增的部分18S小亚基核糖体RNA编码区进行序列分析，结果显示与GenBank中的赖氏艾美耳球虫有93%的同源性，提示为一种新型艾美耳球虫。
