Woodyard Ethan T, Rosser Thomas G, Rush Scott A, McAllister Chris T, Hnida John A, Griffin Matt J
Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39759, USA.
Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39759, USA.
Acta Parasitol. 2021 Jun;66(2):699-705. doi: 10.1007/s11686-020-00317-9. Epub 2021 Jan 2.
The present work aims to morphologically and molecularly characterize an Eimeria sp. recovered from an adult, captive barred owl Strix varia in Mississippi, USA. While the owl was shedding oocysts, no clinical signs of coccidiosis were observed.
Oocysts collected from a barred owl, S. varia were isolated from feces by fecal flotation and characterized by light microscopy. The oocysts were allowed to sporulate in KCrO for 7 days and measured for comparison with previously described Eimeria species. Genomic DNA was extracted from measured oocysts and the cytochrome c oxidase subunit 1 mitochondrial gene was amplified and sequenced for comparison and phylogenetic analyses with publicly available sequence data.
Oocysts collected from S. varia were morphologically and molecularly consistent with all previous accounts of Eimeria megabubonis from the great horned owl Bubo virginianus and morphologically consistent with the only account of Eimeria varia from S. varia. Phylogenetic analyses grouped the E. megabubonis sequence from the present study with previously reported sequences from E. megabubonis.
Morphological and molecular data from oocysts collected in this study reveal S. varia is a host for E. megabubonis. Agreement between current data, morphological and molecular data of E. megabubonis, and morphological data from the original description of E. varia indicates E. varia is a junior synonym of E. megabubonis.
本研究旨在从形态学和分子水平上对从美国密西西比州一只圈养的成年条纹鸮（Strix varia）体内分离出的艾美耳球虫（Eimeria sp.）进行特征描述。在这只鸮排出卵囊期间，未观察到球虫病的临床症状。
从条纹鸮粪便中通过粪便漂浮法分离出卵囊，并通过光学显微镜进行特征描述。将卵囊置于重铬酸钾（KCrO）中孢子化7天，然后进行测量，以便与先前描述的艾美耳球虫种类进行比较。从测量后的卵囊中提取基因组DNA，扩增并测序细胞色素c氧化酶亚基1线粒体基因，以便与公开的序列数据进行比较和系统发育分析。
从条纹鸮收集的卵囊在形态学和分子水平上与之前从大角鸮（Bubo virginianus）分离出的巨型泡状艾美耳球虫（Eimeria megabubonis）的所有记录一致，在形态学上也与唯一一篇关于条纹鸮的变异艾美耳球虫（Eimeria varia）的记录一致。系统发育分析将本研究中的巨型泡状艾美耳球虫序列与先前报道的巨型泡状艾美耳球虫序列归为一组。
本研究收集的卵囊的形态学和分子数据表明，条纹鸮是巨型泡状艾美耳球虫的宿主。当前数据、巨型泡状艾美耳球虫的形态学和分子数据以及变异艾美耳球虫原始描述中的形态学数据之间的一致性表明，变异艾美耳球虫是巨型泡状艾美耳球虫的次异名。
