Woodyard Ethan T, Rush Scott A, Rosser T Graham
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA.
Department of Wildlife, Fisheries & Aquaculture, College of Forest Resources, Mississippi State University, Mississippi State, MS, 39762, USA.
Syst Parasitol. 2019 Sep;96(7):585-594. doi: 10.1007/s11230-019-09867-7. Epub 2019 Jul 22.
While nine nominal species of Eimeria Schneider, 1875 have been described from strigiform birds, molecular sequence data are not available for any of these species. In the present study, oöcysts of a coccidian were isolated by faecal flotation from the lower intestinal contents of an opportunistically collected, recently deceased great horned owl Bubo virginianus (Gmelin), sporulated in potassium dichromate, and subjected to morphological and molecular characterisation. Comparisons of morphological data with previous accounts of Eimeria spp. from owls were consistent with Eimeria megabubonis Upton, Campbell, Weigel & McKown, 1990. Novel molecular data for the 18S ribosomal RNA gene region and the mitochondrial cytochrome c oxidase subunit 1 gene are provided. The results of phylogenetic analysis based on concatenated sequence data from these regions are presented and implications for the evolutionary history of Eimeria are discussed.
虽然自1875年施奈德首次描述艾美耳球虫以来，已从鸮形目鸟类中鉴定出9种艾美耳球虫，但尚未获得这些种类的分子序列数据。在本研究中，通过粪便漂浮法从一只偶然收集的、近期死亡的大角鸮（Bubo virginianus，Gmelin）的下段肠道内容物中分离出一种球虫的卵囊，在重铬酸钾中进行孢子化处理，并进行形态学和分子特征分析。将形态学数据与先前关于鸮类艾美耳球虫种类的描述进行比较，结果与1990年厄普顿、坎贝尔、魏格尔和麦考恩描述的大泡艾美耳球虫（Eimeria megabubonis）一致。本文提供了该球虫18S核糖体RNA基因区域和线粒体细胞色素c氧化酶亚基1基因的新分子数据。展示了基于这些区域串联序列数据的系统发育分析结果，并讨论了其对艾美耳球虫进化历史的影响。
