Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; ABC Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Bioresour Technol. 2017 Apr;230:8-14. doi: 10.1016/j.biortech.2017.01.027. Epub 2017 Jan 16.
This study addresses wet in situ transesterification of microalgae for the production of biodiesel by introducing ethyl acetate as both reactant and co-solvent. Ethyl acetate and acid catalyst are mixed with wet microalgae in one pot and the mixture is heated for simultaneous lipid extraction and transesterification. As a single reactant and co-solvent, ethyl acetate can provide higher FAEE yield and more saccharification of carbohydrates than the case of binary ethanol and chloroform as a reactant and a co-solvent. The optimal yield was 97.8wt% at 114°C and 4.06M catalyst with 6.67mlEtOAC/g dried algae based on experimental results and response surface methodology (RSM). This wet in situ transesterification of microalgae using ethyl acetate doesn't require an additional co-solvent and it also promises more economic benefit as combining extraction and transesterification in a single process.
本研究通过引入乙酸乙酯作为反应物和共溶剂，解决了微藻湿式原位酯交换转化生产生物柴油的问题。乙酸乙酯和酸催化剂与湿微藻在一个锅中混合，然后加热混合物，同时进行脂质提取和酯交换。作为单一的反应物和共溶剂，乙酸乙酯可以比二元乙醇和氯仿作为反应物和共溶剂提供更高的 FAEE 产率和更多的碳水化合物糖化。根据实验结果和响应面法（RSM），在 114°C 和 4.06M 催化剂下，最佳产率为 97.8wt%，每克干藻用 6.67mlEtOAC。这种使用乙酸乙酯的微藻湿式原位酯交换不需要额外的共溶剂，并且由于将提取和酯交换结合在一个单一的过程中，因此有望带来更大的经济效益。
