Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, 518055, China.
State Key Laboratory of Urban Water Resource and Environment (SKLUWRE), Harbin Institute of Technology, Harbin, 150001, China.
World J Microbiol Biotechnol. 2018 Feb 19;34(3):39. doi: 10.1007/s11274-018-2421-z.
Subcritical co-solvents of n-hexane/isopropanol were primarily utilized to extract lipid from wet microalgal pastes of Scenedesmus obliquus. The effects of key operational parameters were investigated, and the optimal parameters were obtained: solvent ratio of n-hexane to isopropanol was 3:2 (V:V), phase ratio of co-solvents to microalgal biomass was 35:1 (mL:g), reactor stirring speed was 900 rpm, extraction time was 60 min. Additional pretreatment with acid, ultrasonic and microwave as well as enhanced subcritical pressure/heating treatments were also applied to further study their effects on lipid extraction. The results showed that the lipid recovery rate with acid pretreatment was 8.6 and 6.2% higher than ultrasonic and microwave pretreatment; the optimum enhanced subcritical condition was 55 °C with atmospheric pressure. Under optimal operating conditions, the lipid and FAME yield were 13.5 and 7.2%, which was 82.6 and 135.1% higher than the traditional method. The results indicated that the subcritical n-hexane/isopropanol extraction process had promising application potential.
正己烷/异丙醇的亚临界共溶剂主要用于从斜生栅藻的湿微藻糊中提取脂质。考察了关键操作参数的影响，得到了最佳参数：正己烷与异丙醇的溶剂比为 3:2（V:V），共溶剂与微藻生物质的相比为 35:1（mL:g），反应器搅拌速度为 900rpm，提取时间为 60min。还采用了酸预处理、超声和微波预处理以及强化亚临界压力/加热处理，以进一步研究它们对脂质提取的影响。结果表明，酸预处理的脂质回收率比超声和微波预处理分别高 8.6%和 6.2%；最佳强化亚临界条件为 55°C 和常压。在最佳操作条件下，脂质和 FAME 的产率分别为 13.5%和 7.2%，比传统方法分别提高了 82.6%和 135.1%。结果表明，亚临界正己烷/异丙醇萃取工艺具有广阔的应用前景。
