Niu Erli, Cai Caiping, Zheng Yongjie, Shang Xiaoguang, Fang Lei, Guo Wangzhen
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Hybrid Cotton R&D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing, 210095, China.
Mol Genet Genomics. 2016 Jun;291(3):1137-54. doi: 10.1007/s00438-016-1169-0. Epub 2016 Jan 30.
Members of the CrRLK1L family, a subgroup of the receptor-like kinase (RLK) gene family, are thought to act as sensors for the integrity of the cell wall and regulators of polar elongation. To better understand the various functions in fiber development, we conducted genome-wide identification and characterization analyses of CrRLK1L family in cotton. Here 44, 40, and 79 CrRLK1L genes were identified from three cotton species: diploid G. raimondii (D5), diploid G. arboreum (A2), and tetraploid G. hirsutum TM-1 (AD1), respectively. The 44 CrRLK1Ls in G. raimondii were anchored to the 12 chromosomes unevenly and were classified into six groups (I-VI), with group II and group IV being further divided into two subgroups (groups IIa and IIb, and IVa and IVb, respectively). These CrRLK1Ls displayed a highly regular pattern of developmental and spatial regulation in cotton. Using the transcriptome data of five chromosomal segment introgression lines (CSILs) and the physical integration of CrRLK1Ls with the quantitative trait loci (QTLs) related to fiber quality traits, we revealed that six CrRLK1L genes were highly associated with fiber development. This study brings new insights into the integrated genome-wide identification of CrRLK1Ls in cotton and provides references for the genetic improvement of cotton fiber.
CrRLK1L家族是类受体激酶（RLK）基因家族的一个亚组，被认为可作为细胞壁完整性的传感器和极性伸长的调节因子。为了更好地了解其在纤维发育中的各种功能，我们对棉花中的CrRLK1L家族进行了全基因组鉴定和特征分析。在此，分别从三种棉花中鉴定出44个、40个和79个CrRLK1L基因：二倍体雷蒙德氏棉（D5）、二倍体亚洲棉（A2）和四倍体陆地棉TM-1（AD1）。雷蒙德氏棉中的44个CrRLK1L基因不均匀地定位在12条染色体上，被分为六组（I-VI），其中第二组和第四组又进一步分为两个亚组（分别为IIa和IIb组，以及IVa和IVb组）。这些CrRLK1L基因在棉花中表现出高度规则的发育和空间调控模式。利用五个染色体片段渐渗系（CSIL）的转录组数据以及CrRLK1L基因与纤维品质性状相关的数量性状位点（QTL）的物理整合，我们揭示了六个CrRLK1L基因与纤维发育高度相关。本研究为棉花中CrRLK1L基因的全基因组综合鉴定带来了新见解，并为棉花纤维的遗传改良提供了参考。
