Han Yehong, Yang Chunliu, Zhou Yang, Han Dandan, Yan Hongyuan
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, College of Public Health, and ‡Key Laboratory of Analytical Science and Technology of Hebei Province & College of Chemistry and Environmental Science, Hebei University , Baoding 071002, China.
J Agric Food Chem. 2017 Mar 1;65(8):1750-1757. doi: 10.1021/acs.jafc.6b03922. Epub 2017 Feb 16.
A new method involving ionic liquid-hybrid molecularly imprinted material-filter solid-phase extraction coupled to high-performance liquid chromatography (IL-HIM-FSPE-HPLC) was developed for the simultaneous isolation and determination of 6-benzyladenine (6-BA) and 4-chlorophenoxyacetic acid (4-CPA) in bean sprouts. Sample preconcentration was performed using a modified filter, with the new IL-HIM as the adsorbent, which shows double adsorption. The first adsorption involves special recognition of molecular imprinting, and the second involves ion exchange and electrostatic attraction caused by the ionic liquid. This method combines the advantages of ionic liquids, hybrid materials, and molecularly imprinted polymers and was successfully applied to determine 6-BA and 4-CPA in bean sprouts. The adsorption of 6-BA to IL-HIM is based on selective imprinted recognition, whereas the adsorption of 4-CPA is mainly dependent on ion-exchange interactions.
建立了一种基于离子液体-混合分子印迹材料-过滤固相萃取结合高效液相色谱法（IL-HIM-FSPE-HPLC）的新方法，用于同时分离和测定豆芽中的6-苄基腺嘌呤（6-BA）和4-氯苯氧乙酸（4-CPA）。使用改良的滤器进行样品预浓缩，以新型离子液体-混合分子印迹材料作为吸附剂，该吸附剂具有双重吸附作用。第一次吸附涉及分子印迹的特异性识别，第二次吸附涉及离子液体引起的离子交换和静电吸引。该方法结合了离子液体、混合材料和分子印迹聚合物的优点，并成功应用于测定豆芽中的6-BA和4-CPA。6-BA对离子液体-混合分子印迹材料的吸附基于选择性印迹识别，而4-CPA的吸附主要依赖于离子交换相互作用。
