Tiwari Apoorv, Avashthi Himanshu, Jha Richa, Srivastava Ambuj, Kumar Garg Vijay, Wasudev Ramteke Pramod, Kumar Anil
Department of Molecular Biology & Genetic Engineering, CBSH, G. B. Pant University of Agriculture & Technology, Pantnagar-263145,Uttarakhand, Bharat (India).
Department of Computational Biology & Bioinformatics, JSBB, Sam Higginbottom Institute of Agriculture,Technology and Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
Bioinformation. 2016 Jun 15;12(3):156-164. doi: 10.6026/97320630012156. eCollection 2016.
Lipoxygenase-1 (LOX-1) protein provides defense against pests and pathogens and its presence have been positively correlated with plant resistance against pathogens. Linoleate is a known substrate of lipoxygenase and it induces necrosis leading to the accumulation of isoflavonoid phytoalexins in plant leaves. Therefore, it is of interest to study the structural features of LOX-1 from Finger millet. However, the structure ofLOX-1 from Finger millet is not yet known. A homology model of LOX-1 from Finger millet is described. Domain architecture study suggested the presence of two domains namely PLAT (Phospho Lipid Acyl Transferase) and lipoxygenase. Molecular docking models of linoleate with lipoxygenase from finger millet, rice and sorghum are reported. The features of docked models showed that finger millet have higher pathogen resistance in comparison to other cereal crops. This data is useful for the molecular cloning of fulllength LOX-1 gene for validating its role in improving plant defense against pathogen infection and for various other biological processes.
脂氧合酶-1（LOX-1）蛋白可抵御害虫和病原体，其存在与植物对病原体的抗性呈正相关。亚油酸是脂氧合酶的已知底物，它会诱导坏死，导致植物叶片中异黄酮植保素的积累。因此，研究龙爪稷中LOX-1的结构特征具有重要意义。然而，龙爪稷中LOX-1的结构尚不清楚。本文描述了龙爪稷中LOX-1的同源模型。结构域架构研究表明存在两个结构域，即PLAT（磷脂酰基转移酶）和脂氧合酶。报道了亚油酸与龙爪稷、水稻和高粱脂氧合酶的分子对接模型。对接模型的特征表明，与其他谷类作物相比，龙爪稷具有更高的病原体抗性。这些数据对于全长LOX-1基因的分子克隆很有用，可用于验证其在改善植物对病原体感染的防御以及其他各种生物学过程中的作用。
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