Saari Shafikha Mohd, Basri Dayang Fredalina, Budin Siti Balkis, Warif Nor Malia Abd
School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, 50300 Kuala Lumpur, Malaysia.
Saudi J Biol Sci. 2017 Feb;24(2):320-323. doi: 10.1016/j.sjbs.2015.09.032. Epub 2015 Oct 9.
Type 1 diabetes mellitus is a chronic disease characterized by lack of insulin production. Immune mechanisms are implicated in the pathogenesis of Type 1 diabetes. (CO) fruits and leaves have been shown to possess high antioxidant activity. This study was conducted to evaluate the effects of CO leaves aqueous extract on the blood glucose and T lymphocyte population in the spleen of streptozotocin (STZ)-induced diabetic rats. Nineteen male Sprague-Dawley rats were randomly divided into three groups: normal, diabetic control and CO treated diabetic groups. Diabetes was induced by a single intraperitoneal injection of 65 mg STZ/kg body weight. The extract of CO leaves was administered orally by force feeding daily at the dose of 300 mg/kg for 28 days. The rats were sacrificed at the end of the study and the spleen was harvested for flow cytometry analysis. The results showed a significant decrease in body weight of diabetic and CO treated diabetic groups compared with the normal group ( < 0.05). The fasting blood glucose level of CO treated diabetic group was significantly lower than the diabetic group ( < 0.05). Diabetic and CO treated diabetic groups showed a significant increase in the percentage of spleen CD3 CD4 T lymphocytes ( < 0.05) when compared with the normal group. However, there was no significant difference in the percentage of spleen CD3 CD8 T lymphocytes among all experimental groups. The finding suggested that an aqueous extract of CO leaves has the ability to reduce blood glucose levels in diabetic rats.
1型糖尿病是一种以胰岛素分泌不足为特征的慢性疾病。免疫机制与1型糖尿病的发病机制有关。（CO）的果实和叶子已被证明具有高抗氧化活性。本研究旨在评估CO叶水提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠血糖和脾脏T淋巴细胞群体的影响。将19只雄性Sprague-Dawley大鼠随机分为三组：正常组、糖尿病对照组和CO治疗糖尿病组。通过腹腔注射65mg STZ/kg体重单次诱导糖尿病。CO叶提取物以300mg/kg的剂量每日强制灌胃给药28天。在研究结束时处死大鼠，收获脾脏用于流式细胞术分析。结果显示，与正常组相比，糖尿病组和CO治疗糖尿病组的体重显著降低（P<0.05）。CO治疗糖尿病组的空腹血糖水平显著低于糖尿病组（P<0.05）。与正常组相比，糖尿病组和CO治疗糖尿病组脾脏CD3 CD4 T淋巴细胞百分比显著增加（P<0.05）。然而，所有实验组脾脏CD3 CD8 T淋巴细胞百分比无显著差异。该发现表明，CO叶水提取物具有降低糖尿病大鼠血糖水平的能力。
