Shahsavan Shadi, Nobakht Maliheh, Rastegar-Lari Abdolaziz, Owlia Parviz, Bakhshi Bita
Antimicrobial Resistance Research Center, Rasul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Antimicrobial Resistance Research Center, Rasul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran; Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2016 Oct;8(5):298-306.
Strains of spp. can cause shigellosis, or bacillary dysentery. that is a public health problem worldwide. The aim of this study was to describe the population structure and genetic relatedness of multidrug resistant and isolated during a one year period from children with diarrhea in Tehran, Iran.
A total of 70 spp. were detected during the study period. Twenty MDR isolates of spp. were randomly selected and used in this study. Bacterial identification was performed by conventional biochemical and serological and confirmed by molecular method. After antimicrobial susceptibility testing, we used Multilocus sequence typing (MLST) for subtyping isolates.
We found 14 and 6 isolates. Results of MLST showed five sequence types (ST) (145, 152, 241, 245, 1502) and BURST analysis revealed the largest number of single locus variant (SLV) and highest frequency (FREQ) for ST152. ST 152 with nine members was predicted as the founder by BURST. Frequency for ST 1502 and ST 245 was four isolates and the least frequency was seen for ST 241 and 145 with one and two members, respectively. ST 145 and ST 245 were described as singletons in BURST. All isolates with ST145 and ST245 were identified as
Annual Multi locus sequence typing of MDR would help us in better understanding of dominant species and comparing our results with the same studies in other countries especially our neighbor countries in source tracking purposes.
宋内志贺氏菌菌株可引起志贺氏菌病或杆菌性痢疾，这是一个全球性的公共卫生问题。本研究的目的是描述在伊朗德黑兰一年内从腹泻儿童中分离出的多重耐药宋内志贺氏菌和痢疾志贺氏菌的种群结构及遗传相关性。
在研究期间共检测到70株志贺氏菌属菌株。随机选择20株多重耐药志贺氏菌属菌株用于本研究。通过传统生化和血清学方法进行细菌鉴定，并通过分子方法进行确认。在进行抗菌药物敏感性测试后，我们使用多位点序列分型（MLST）对分离株进行亚型分析。
我们发现了14株宋内志贺氏菌和6株痢疾志贺氏菌分离株。MLST结果显示有五种序列类型（ST）（145、152、241、245、1502），BURST分析显示ST152的单一位点变体（SLV）数量最多且频率（FREQ）最高。BURST预测有九个成员的ST152为始祖型。ST1502和ST245的频率为四个分离株，ST241和145的频率最低，分别为一个和两个成员。ST145和ST245在BURST中被描述为单例。所有ST145和ST245的分离株均被鉴定为[此处原文缺失具体内容]
对多重耐药志贺氏菌进行年度多位点序列分型将有助于我们更好地了解优势菌种，并将我们的结果与其他国家特别是邻国的同类研究进行比较，以用于溯源目的。
