Ninomiya Satoshi, Yoshimura Kentaro, Chen Lee Chuin, Takeda Sen, Hiraoka Kenzo
Interdisciplinary Graduate School, University of Yamanashi.
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi.
Mass Spectrom (Tokyo). 2017;6(1):A0053. doi: 10.5702/massspectrometry.A0053. Epub 2017 Jan 27.
Tissue samples from renal cell carcinoma patients were analyzed by electrospray droplet ion beam-induced secondary ion mass spectrometry (EDI/SIMS). Positively- and negatively-charged secondary ions were measured for the cancerous and noncancerous regions of the tissue samples. Although specific cancerous species could not be found in both the positive and negative secondary ion spectra, the spectra of the cancerous and noncancerous tissues presented different trends. For instance, in the / range of 500-800 of the positive secondary ion spectra for the cancerous tissues, the intensities for several / values were lower than those of the /+2 peaks (indicating one double bond loss for the species), whereas, for the noncancerous tissues, the inverse trend was obtained. The tandem mass spectrometry (MS/MS) was also performed on the tissue samples using probe electrospray ionization (PESI), and some molecular ions produced by PESI were found to be fragmented into the ions observed in EDI/SIMS analysis. When the positive secondary ion spectra produced by EDI/SIMS were analyzed by principal component analysis, the results for cancerous and noncancerous tissues were separated. The EDI/SIMS method can be applied to distinguish between a cancerous and a noncancerous area with high probability.
采用电喷雾液滴离子束诱导二次离子质谱法（EDI/SIMS）对肾细胞癌患者的组织样本进行分析。对组织样本的癌性区域和非癌性区域测量了带正电荷和负电荷的二次离子。尽管在正、负离子二次离子谱中均未发现特定的癌性物质，但癌组织和非癌组织的谱图呈现出不同的趋势。例如，在癌组织正二次离子谱的500 - 800范围内，几个值的强度低于/+2峰的强度（表明该物质失去一个双键），而对于非癌组织，则得到相反的趋势。还使用探针电喷雾电离（PESI）对组织样本进行了串联质谱分析（MS/MS），发现PESI产生的一些分子离子会碎裂成EDI/SIMS分析中观察到的离子。当通过主成分分析对EDI/SIMS产生的正二次离子谱进行分析时，癌组织和非癌组织的结果得以区分。EDI/SIMS方法很有可能用于区分癌性区域和非癌性区域。
