Yoshimura Kentaro, Chen Lee Chuin, Asakawa Daiki, Hiraoka Kenzo, Takeda Sen
Department of Anatomy and Cell Biology, Interdisciplinary School of Medicine and Engineering, The University of Yamanashi, 1110 Shimo-Kateau, Chuo, Yamanashi 409-3898, Japan.
J Mass Spectrom. 2009 Jun;44(6):978-85. doi: 10.1002/jms.1576.
Probe electrospray ionization (PESI) is a recently developed technique that uses a fine solid needle as a probe for sampling biological materials. In this study, we quantified the volume of liquid sample picked up by the solid needle with the tip diameter of approximately 700 nm and the apex angle of approximately 60 degrees. The amounts of low-viscosity samples (rat urine) loaded on the tip of the needle by a single stroke were 0.35 +/- 0.09 pl. Interestingly, the amount of liquid adhered to the tip did not significantly depend on the protein concentration, but viscosity and surface tension of the sample. Under these conditions, we successfully obtained mass spectra for each biological sample.
探针电喷雾电离（PESI）是一种最近开发的技术，它使用一根细的实心针作为探针来采集生物材料。在本研究中，我们对尖端直径约700纳米、顶角约60度的实心针所采集的液体样品体积进行了量化。单次进样加载到针尖上的低粘度样品（大鼠尿液）量为0.35±0.09皮升。有趣的是，附着在针尖上的液体量并不显著取决于蛋白质浓度，而是取决于样品的粘度和表面张力。在这些条件下，我们成功获得了每个生物样品的质谱图。
