Sokolov Igor, Dokukin Maxim E
Department of Mechanical Engineering, Tufts University, 177 College Ave., Medford, MA, 02155, USA.
Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
Methods Mol Biol. 2017;1530:229-245. doi: 10.1007/978-1-4939-6646-2_13.
Fractal analysis of the cell surface is a rather sensitive method which has been recently introduced to characterize cell progression toward cancer. The surface of fixed and freeze-dried cells is imaged with atomic force microscopy (AFM) modality in ambient conditions. Here we describe the method to perform the fractal analysis specifically developed for the AFM images. Technical details, potential difficulties, points of special attention are described.
细胞表面的分形分析是一种相当灵敏的方法，该方法最近被引入用于表征细胞向癌症发展的进程。在环境条件下，使用原子力显微镜（AFM）模式对固定和冻干细胞的表面进行成像。在此，我们描述了专门针对AFM图像开发的分形分析方法。文中介绍了技术细节、潜在困难及特别需要注意的要点。
