Banerjee Shashwat S, Khobragade Vrushali, Khandare Jayant
Maharashtra Institute of Medical Education and Research Medical College, Talegaon Dabhade, Pune, 410507, India.
Actorius Innovations and Research, 100 NCL Innovation Centre, Pune, 411008, India.
Methods Mol Biol. 2017;1530:271-281. doi: 10.1007/978-1-4939-6646-2_16.
Detection of circulating tumor cells (CTCs) in the blood circulation holds immense promise as it predicts the overall probability of patient survival. Therefore, CTC-based technologies are gaining prominence as a "liquid biopsy" for cancer diagnostics and prognostics. Here, we describe the design and synthesis of two distinct multicomponent magnetic nanosystems for rapid capture and detection of CTCs. The multifunctional Magneto-Dendrimeric Nano System (MDNS) composed of an anchoring dendrimer that is conjugated to multiple agents such as near infrared (NIR) fluorescent cyanine 5 NHS (Cy5), glutathione (GSH), transferrin (Tf), and iron oxide (FeO) magnetic nanoparticle (MNP) for simultaneous tumor cell-specific affinity, multimodal high resolution confocal imaging, and cell isolation. The second nanosystem is a self-propelled microrocket that is composed of carbon nanotube (CNT), chemically conjugated with targeting ligand such as transferrin on the outer surface and FeO nanoparticles in the inner surface. The multicomponent nanosystems described here are highly efficient in targeting and isolating cancer cells thus benefiting early diagnosis and therapy of cancer.
检测血液循环中的循环肿瘤细胞（CTC）具有巨大的前景，因为它可以预测患者的总体生存概率。因此，基于CTC的技术作为一种用于癌症诊断和预后的“液体活检”正日益受到关注。在此，我们描述了两种不同的多组分磁性纳米系统的设计与合成，用于快速捕获和检测CTC。多功能磁树状纳米系统（MDNS）由一个锚定树状大分子组成，该树状大分子与多种试剂共轭，如近红外（NIR）荧光花菁5 NHS（Cy5）、谷胱甘肽（GSH）、转铁蛋白（Tf）和氧化铁（FeO）磁性纳米颗粒（MNP），用于同时实现肿瘤细胞特异性亲和力、多模态高分辨率共聚焦成像和细胞分离。第二种纳米系统是一种自驱动微火箭，它由碳纳米管（CNT）组成，在外表面化学共轭有靶向配体如转铁蛋白，在内表面有FeO纳米颗粒。这里描述的多组分纳米系统在靶向和分离癌细胞方面非常高效，从而有利于癌症的早期诊断和治疗。
