Paro Autumn D, Shanmugam Ilanchezhian, van de Ven Anne L
Department of Chemical Engineering, Northeastern University, Boston, MA, USA.
Nanomedicine Science & Technology Center, Northeastern University, Boston, MA, USA.
Methods Mol Biol. 2017;1530:391-401. doi: 10.1007/978-1-4939-6646-2_25.
Metallic nanoparticles with a high atomic number release Auger electrons in response to external beam X-ray radiation. When these nanoparticles are selectively delivered to tumors, they have the potential to locally enhance the effects of radiation therapy. Optimizing the therapeutic efficacy of these nanoparticles, however, remains a challenging and time-consuming task. Here we describe three different assays that can be used to experimentally quantify and optimize the in vitro therapeutic efficacy of nanoparticle-mediated X-ray radiation enhancement. These include an IC50 extended dose response curve, clonogenic cell survival assay, and immunoblotting. Collectively, these assays provide information about whether a given nanoparticle provides radiosensitization, the extent of the radiosensitization, and the potential mechanism of radiosensitization.
具有高原子序数的金属纳米颗粒会响应外部束流X射线辐射释放俄歇电子。当这些纳米颗粒被选择性地递送至肿瘤时，它们有可能局部增强放射治疗的效果。然而，优化这些纳米颗粒的治疗效果仍然是一项具有挑战性且耗时的任务。在此，我们描述了三种不同的检测方法，可用于通过实验量化和优化纳米颗粒介导的X射线辐射增强的体外治疗效果。这些方法包括IC50扩展剂量反应曲线、克隆形成细胞存活检测和免疫印迹。总体而言，这些检测方法提供了有关给定纳米颗粒是否具有放射增敏作用、放射增敏的程度以及放射增敏潜在机制的信息。
