蓝莓花色苷摄入期间人体尿液中的类黄酮代谢物。
Flavonoid Metabolites in Human Urine during Blueberry Anthocyanin Intake.
机构信息
Kentville Research and Development Centre, Agriculture and Agri-Food Canada , 32 Main Street, Kentville, Nova Scotia B4N 1J5, Canada.
Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences , No. 4899 Juye Street, Changchun 130112, China.
出版信息
J Agric Food Chem. 2017 Mar 1;65(8):1582-1591. doi: 10.1021/acs.jafc.6b05455. Epub 2017 Feb 17.
The human health benefits of anthocyanins (Anc) and other flavonoids are widely recognized. However, the flavonoid-based urinary metabolites arising in vivo after Anc intake are not well described. Human (n = 17) urine was collected while blueberry juice (BJ) was consumed daily for 28 days and once after a 7 day washout. MS/MS scanning of 664 urine samples for 18 parent Anc (PAnc) and 42 predicted Anc metabolites (AncM) yielded 371 products (i.e., MS/MS × retention time (RT)). Flavonoid-based AncM, which were likely underestimated, were almost 20 times more abundant than PAnc. Together, PAnc and AncM accounted for about 1% of the daily Anc dose. Aglycone forms were >94% of the total. Cluster analysis of the 371 Anc identified about 55 major Anc that contributed about 80% to the total Anc. The abundance of flavonoid-based Anc-derived products in the gastrointestinal tract could contribute to the health benefits of Anc-rich berries.
花色苷（Anc）和其他类黄酮对人类健康的益处已得到广泛认可。然而，Anc 摄入后在体内产生的基于类黄酮的尿代谢产物尚未得到很好的描述。17 名志愿者连续 28 天每天饮用蓝莓汁（BJ），并在 7 天洗脱期后再次饮用，在此期间收集他们的尿液。对 664 个尿液样本进行 664 次 MS/MS 扫描，以检测 18 种母体Anc（PAnc）和 42 种预测Anc 代谢物（AncM），共产生 371 种产物（即 MS/MS×保留时间（RT））。AncM 是基于类黄酮的AncM，其含量可能被低估，其丰度几乎是 PAnc 的 20 倍。PAnc 和 AncM 共同占每日 Anc 剂量的约 1%。总共有超过 94%的物质为糖苷形式。对 371 种Anc 的聚类分析确定了大约 55 种主要Anc，它们约占总Anc 的 80%。消化道中基于类黄酮的Anc 衍生产物的丰度可能有助于富含Anc 的浆果发挥其健康益处。
Zotero 插件