Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan.
Laboratory for Integrative Omics, RIKEN Quantitative Biology Center (QBiC), 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan and Laboratory for Immunogenetics, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan and JST PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan.
Lab Chip. 2017 Feb 14;17(4):647-652. doi: 10.1039/c6lc01313a.
Herein we examined the extent of replicability of the PDMS microchamber device transferred from the master mold with complex 3D structures fabricated via micro stereolithography. Due to the elastomeric properties of PDMS, the reversely tapered micromold, with the diameter ratio of ∼5 from the largest to the narrowest part, was precisely transferred without breaking. We obtained the mathematical model to estimate the stress exerted on the mold during the demolding process. Finally, we tested the applicability of this unusual microchamber for single-cell trapping and an enzyme assay.
在这里，我们研究了通过微立体光刻技术制造的具有复杂 3D 结构的主模具转移到 PDMS 微腔装置的可重复性程度。由于 PDMS 的弹性特性，反向锥形微模具能够精确地转移而不会破裂，其直径比从最大到最窄部分约为 5。我们获得了用于估计脱模过程中模具上所受应力的数学模型。最后，我们测试了这种不寻常的微腔用于单细胞捕获和酶测定的适用性。
