一种新型 CdTe 量子点探针放大共振光散射信号来检测 microRNA-122。
A novel CdTe quantum dots probe amplified resonance light scattering signals to detect microRNA-122.
机构信息
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
出版信息
Talanta. 2017 Apr 1;165:659-663. doi: 10.1016/j.talanta.2017.01.020. Epub 2017 Jan 7.
We report a rapid and facile resonance light scattering (RLS) technique that utilizes CdTe quantum dots (CdTe QDs) probe to detect microRNA-122. The RLS sensor is ingeniously designed with P1 and P2, two cDNA sequence probes with partially complementary sequences to miRNA-122. The amine-modified P1 and P2 are coupled to the surface of QDs to form functional QDs-P1 and QDs-P2 conjugates, which are collectively referred to as QDs-P. The cDNAs hybridize with the target miRNA to rapidly induce the self-assembly of QDs probe and change RLS intensity. The proposed technique can detect miRNA-122 within 40min. RLS intensity is enhanced in proportion with miRNA-122 concentrations of 0.16-4.80nM and has a low detection limit of 9.4pM. In addition, the assay satisfactorily detects miRNAs in human serum samples. Thus, the assay has considerable potential for the analysis of other interesting tumor makers.
我们报告了一种快速简便的共振光散射（RLS）技术，该技术利用碲化镉量子点（CdTe QDs）探针来检测 microRNA-122。RLS 传感器巧妙地设计了 P1 和 P2 两个 cDNA 序列探针，它们与 miRNA-122 具有部分互补序列。经氨基修饰的 P1 和 P2 与 QDs 表面偶联，形成具有功能的 QDs-P1 和 QDs-P2 缀合物，统称为 QDs-P。cDNA 与靶 miRNA 杂交，可快速诱导 QDs 探针自组装并改变 RLS 强度。该技术可在 40min 内检测 miRNA-122。RLS 强度与 miRNA-122 浓度在 0.16-4.80nM 范围内呈比例增强，检测限低至 9.4pM。此外，该测定法可令人满意地检测人血清样本中的 miRNAs。因此，该测定法在分析其他感兴趣的肿瘤标志物方面具有很大的潜力。
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