Phylogenetic analysis of isolated from broilers with colibacillosis based on gene sequences.

isolates from chickens with colibacillosis were assigned to phylogenetic groups based on multiplex polymerase chain reaction (PCR) and antibacterial resistance of belonging to these groups was examined. Furthermore, the A gene of isolates was sequenced and a phylogenetic tree was generated. A total of 84 isolates were grouped using multiplex PCR of TSPE4.C2, A, A, and A molecular markers. Four phylogenetic groups were identified with strains divided as follows: 16 in group A (19.05%), 17 in group B1 (20.24%), 23 in group B2 (27.38%), and 28 in group D (33.33%). isolates belonging to phylogenetic groups B2 and D were resistant to Soltrim and Flumequine unlike the majority of isolates that belonged to groups A and B1, and which were susceptible to these antibiotics. The phylogenetic results based on gene sequences from multiplex PCR revealed that phylogenetic grouping was in accordance with the clusters obtained in the phylogenetic tree. In conclusion, the comparative sequence analysis of sequences provides a firm framework for an accurate classification of and related taxa and may constitute a pertinent phylogenetic marker for