Shamsi Hamid, Mardani Karim, Ownagh Abdolghaffar
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Shamsi, Mardani); Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Ownagh).
Can J Vet Res. 2017 Jan;81(1):28-32.
isolates from chickens with colibacillosis were assigned to phylogenetic groups based on multiplex polymerase chain reaction (PCR) and antibacterial resistance of belonging to these groups was examined. Furthermore, the A gene of isolates was sequenced and a phylogenetic tree was generated. A total of 84 isolates were grouped using multiplex PCR of TSPE4.C2, A, A, and A molecular markers. Four phylogenetic groups were identified with strains divided as follows: 16 in group A (19.05%), 17 in group B1 (20.24%), 23 in group B2 (27.38%), and 28 in group D (33.33%). isolates belonging to phylogenetic groups B2 and D were resistant to Soltrim and Flumequine unlike the majority of isolates that belonged to groups A and B1, and which were susceptible to these antibiotics. The phylogenetic results based on gene sequences from multiplex PCR revealed that phylogenetic grouping was in accordance with the clusters obtained in the phylogenetic tree. In conclusion, the comparative sequence analysis of sequences provides a firm framework for an accurate classification of and related taxa and may constitute a pertinent phylogenetic marker for
对来自患有大肠杆菌病的鸡的分离株，基于多重聚合酶链反应（PCR）将其归入系统发育组，并检测这些组的分离株的抗菌耐药性。此外，对分离株的A基因进行测序并构建系统发育树。使用TSPE4.C2、A、A和A分子标记的多重PCR对总共84株分离株进行分组。鉴定出四个系统发育组，菌株划分如下：A组16株（19.05%），B1组17株（20.24%），B2组23株（27.38%），D组28株（33.33%）。与大多数属于A组和B1组且对这些抗生素敏感的分离株不同，属于系统发育组B2和D的分离株对磺胺甲氧嗪和氟甲喹耐药。基于多重PCR的基因序列的系统发育结果表明，系统发育分组与系统发育树中获得的聚类一致。总之，序列的比较分析为准确分类和相关分类群提供了坚实的框架，并且可能构成一个相关的系统发育标记用于
