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巴西家禽屠宰场肉鸡分离株的系统发育群

Phylogenetic Group of Isolates from Broilers in Brazilian Poultry Slaughterhouse.

作者信息

Coura Fernanda M, Diniz Soraia A, Silva Marcos X, Arcebismo Thiago L M, Minharro Silvia, Feitosa Adriana C F, Lage Andrey P, Knöbl Terezinha, Mussi Jamili Maria Suhet, Heinemann Marcos B

机构信息

Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, No. 6627, 30123-970 Belo Horizonte, MG, Brazil.

Escola de Medicina Veterinária e Zootecnia, Inspeção e Tecnologia de Carnes e Derivados, Universidade Federal do Tocantins (UFT), BR-153, Km 112, 77804-970 Araguaína, TO, Brazil.

出版信息

ScientificWorldJournal. 2017;2017:5898701. doi: 10.1155/2017/5898701. Epub 2017 Oct 10.

Abstract

The aim of the study was to determine the phylogenetic groups of strains isolated from seemingly healthy broiler and broiler condemned suspected of colibacillosis in a Brazilian slaughterhouse. Samples from respiratory tract and edible giblets (liver and heart) of broilers with and without macroscopic lesions of colibacillosis were collected at slaughter. There were 84 strains isolated from broilers condemned of which 11 were obtained from swabs of the heart, 7 from the liver, and 66 from the respiratory tract. Of the 53 strains isolated from broilers not condemned, 5 were isolated from the heart, 4 from the liver, and 44 from the respiratory tract. strains were tested via PCR for phylogenetic groups A, B1, B2, C, D, E, and F. Phylogroups A and B1 were the most common phylogroups of obtained from healthy and sick-appearing broiler carcasses. The results of the study showed that phylogroups B2 and E were associated with the heart samples and phylogroup A was associated with respiratory tract samples, phylogroup B1 with not condemned carcass, and phylogroup D with liver samples.

摘要

本研究的目的是确定从巴西一家屠宰场看似健康的肉鸡以及疑似患有大肠杆菌病而被拒收的肉鸡中分离出的菌株的系统发育群。在屠宰时,采集了有和没有大肠杆菌病宏观病变的肉鸡呼吸道和可食用内脏(肝脏和心脏)的样本。从被拒收的肉鸡中分离出84株菌株,其中11株来自心脏拭子,7株来自肝脏,66株来自呼吸道。从未被拒收的肉鸡中分离出的53株菌株中,5株来自心脏,4株来自肝脏,44株来自呼吸道。通过PCR对菌株进行A、B1、B2、C、D、E和F系统发育群的检测。系统发育群A和B1是从健康和看似患病的肉鸡胴体中获得的最常见的系统发育群。研究结果表明,系统发育群B2和E与心脏样本相关,系统发育群A与呼吸道样本相关,系统发育群B1与未被拒收的胴体相关,系统发育群D与肝脏样本相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec5b/5654288/718745eaf1ed/TSWJ2017-5898701.001.jpg

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