Park Hyeok, Lee Eun-Hwa, Ka Kang-Hyeon, Eom Ahn-Heum
Department of Biology Education, Korea National University of Education, Cheongju 28173, Korea.
Division of Wood Chemistry & Microbiology, Korea Forest Research Institute, Seoul 02455, Korea.
Mycobiology. 2016 Dec;44(4):277-282. doi: 10.5941/MYCO.2016.44.4.277. Epub 2016 Dec 31.
In this study, we collected rhizosphere soils and root samples from a post-mining area and a natural forest area in Jecheon, Korea. We extracted spores of arbuscular mycorrhizal fungi (AMF) from rhizospheres, and then examined the sequences of 18S rDNA genes of the AMF from the collected roots of plants. We compared the AMF communities in the post-mining area and the natural forest area by sequence analysis of the AMF spores from soils and of the AMF clones from roots. Consequently, we confirmed that the structure of AMF communities varied between the post-mining area and the natural forest area and showed significant relationship with heavy metal contents in soils. These results suggest that heavy metal contamination by mining activity significantly affects the AMF community structure.
在本研究中,我们从韩国堤川市的一个采矿后区域和一个天然林区采集了根际土壤和根系样本。我们从根际提取了丛枝菌根真菌(AMF)的孢子,然后检测了从采集的植物根系中提取的AMF的18S rDNA基因序列。我们通过对土壤中AMF孢子和根系中AMF克隆的序列分析,比较了采矿后区域和天然林区的AMF群落。结果,我们证实了AMF群落结构在采矿后区域和天然林区之间存在差异,并且与土壤中的重金属含量存在显著关系。这些结果表明,采矿活动造成的重金属污染显著影响了AMF群落结构。