Onaga H, Togo M, Kudo Y, Motohashi T, Ishii T
Nippon Institute for Biological Science, Tokyo, Japan.
Vet Parasitol. 1989 Oct;33(3-4):199-205. doi: 10.1016/0304-4017(89)90129-5.
Nine thousand commercial breeder chicks (Chankee) reared in a floor pen were exposed to restricted numbers of Eimeria tenella and E. necatrix oocysts to confer immunity. Antibody induction in these chicks was examined by the enzyme-linked immunosorbent assay (ELISA) with antigen prepared from E. tenella oocysts. The oocyst excretion pattern demonstrated recycled infections which continued in these chicks for greater than or equal to 22 days after exposure. Antibody levels in their sera, as determined by the mean absorbence values in ELISA, increased gradually up to 38 days post-inoculation. Mean absorbence values of sera from control chicks remained at a low level. When infected and control chicks were challenged with the two species of coccidia, the test chicks were protected against both species. The antibody level did not change for 8 days in the challenge groups, while in the control chicks, absorbence in ELISA rose significantly and the mean absorbence value was higher than that in immunized chicks. Some factors which influence the results of ELISA are considered and the applicability of this method to measuring immunity against coccidiosis in chickens is discussed.
将9000只饲养在地面围栏中的商品种鸡（Chankee）暴露于数量有限的柔嫩艾美耳球虫和毒害艾美耳球虫卵囊中以诱导免疫。用从柔嫩艾美耳球虫卵囊中制备的抗原，通过酶联免疫吸附测定（ELISA）检测这些雏鸡体内抗体的诱导情况。卵囊排泄模式显示出反复感染，在暴露后这些雏鸡中这种感染持续了大于或等于22天。通过ELISA中的平均吸光度值测定，它们血清中的抗体水平在接种后38天内逐渐升高。对照雏鸡血清的平均吸光度值维持在较低水平。当用这两种球虫对感染组和对照组雏鸡进行攻毒时，试验雏鸡对这两种球虫均有抵抗力。攻毒组的抗体水平在8天内没有变化，而在对照雏鸡中，ELISA中的吸光度显著上升，且平均吸光度值高于免疫雏鸡。文中考虑了一些影响ELISA结果的因素，并讨论了该方法在检测鸡对球虫病免疫力方面的适用性。
