Onaga H, Saeki H, Hoshi S, Ueda S
Avian Dis. 1986 Oct-Dec;30(4):658-61.
A simplified enzyme-linked immunosorbent assay (ELISA) was used to survey broiler chickens for coccidial infection. With an antigen derived from Eimeria tenella oocysts and a single serum dilution of 1:100, the absorbance values were directly used for the evaluation of antibody levels. When the detection level, determined with sera from specific-pathogen-free chickens, was used as the negative standard, randomly selected sera from broiler chickens essentially were negative. Sera were periodically collected from two flocks of commercial broilers reared under medication, and their ELISA titers were compared with their excretion of oocysts. When the oocyst isolation rate indicated that infection was severe, a substantial number of chickens showed high absorbance values in ELISA. These results indicate that coccidial infections in the field can be detected serologically with ELISA.
采用一种简化的酶联免疫吸附测定法（ELISA）对肉鸡的球虫感染情况进行调查。使用源自柔嫩艾美耳球虫卵囊的抗原以及1:100的单一血清稀释度，吸光度值直接用于评估抗体水平。当以无特定病原体鸡的血清所确定的检测水平作为阴性标准时，随机选取的肉鸡血清基本为阴性。定期从两组用药饲养的商品肉鸡中采集血清，并将其ELISA效价与其卵囊排泄情况进行比较。当卵囊分离率表明感染严重时，大量鸡只在ELISA中显示出高吸光度值。这些结果表明，可通过ELISA血清学检测田间的球虫感染情况。
