Norpoth K, Müller G
Z Gesamte Hyg. 1989;35(8):487-90.
The determination of mercapturic acids in urine samples of employees exposed to carcinogenic substances requires highly sensitive and specific methods such as GC/MS or HPLC/Fluorimetry. We applied GC/MS to the analysis of thiodiglycolic acid, which is an indicator metabolite of vinyl chloride exposure and of S-phenylmercapturic acid which can be used to control benzene exposure. S-cyanoethylmercapturic acid appears as a degradation product during acrylonitrile metabolism. This metabolite can be determined by HPLC combined with a sulfur specific fluorimetric detector. This procedure which is of general use offers the possibility to improve the biological monitoring in case of exposures to carcinogenic substances.
对接触致癌物质的员工尿液样本中硫醇尿酸的测定需要高灵敏度和特异性的方法，如气相色谱/质谱联用仪（GC/MS）或高效液相色谱/荧光分析法（HPLC/荧光法）。我们应用气相色谱/质谱联用仪分析硫代二甘醇酸，它是氯乙烯接触的指示性代谢物，以及S-苯基硫醇尿酸，可用于监测苯接触情况。S-氰基乙基硫醇尿酸是丙烯腈代谢过程中的降解产物。这种代谢物可以通过高效液相色谱结合硫特异性荧光检测器来测定。这种通用方法为改善致癌物质接触情况下的生物监测提供了可能性。
