Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatologı́a, Facultad de Farmacia, University of Salamanca , Campus Miguel de Unamuno, E-37007 Salamanca, Spain.
J Agric Food Chem. 2017 Aug 9;65(31):6359-6368. doi: 10.1021/acs.jafc.6b05278. Epub 2017 Feb 13.
The determination of the detailed flavanol composition in food matrices is not a simple task because of the structural similarities of monomers and, consequently, oligomers and polymers. The aim of this study was the development and validation of an HPLC-MS/MS-multiple reaction monitoring (MRM) method that would allow the accurate and precise quantification of catechins, gallocatechins, and oligomeric proanthocyanidins. The high correlation coefficients of the calibration curves (>0.993), the recoveries not statistically different from 100%, the good intra- and interday precisions (<5%), and the LOD and LOQ values, low enough to quantify flavanols in grapes, are good results from the method validation procedure. Its usefulness has also been tested by determining the detailed composition of Vitis vinifera L. cv. Rufete grapes. Seventy-two (38 nongalloylated and 34 galloylated) and 53 (24 procyanidins and 29 prodelphinidins) flavanols have been identified and quantified in grape seed and grape skin, respectively. The use of HCA and PCA on the detailed flavanol composition has allowed differentiation among Rufete clones.
在食品基质中确定详细的黄烷醇组成并不是一项简单的任务，因为单体的结构相似，因此寡聚物和聚合物也是如此。本研究的目的是开发和验证一种 HPLC-MS/MS-多重反应监测 (MRM) 方法，该方法能够准确、精确地定量儿茶素、没食子儿茶素和寡聚原花青素。校准曲线的高相关系数（>0.993）、回收率与 100%无统计学差异、良好的日内和日间精密度（<5%）以及检出限和定量限值足够低，足以定量葡萄中的黄烷醇，这些都是方法验证程序的良好结果。通过测定葡萄(Vitis vinifera L. cv. Rufete)的详细成分，也验证了该方法的有用性。在葡萄籽和葡萄皮中分别鉴定和定量了 72 种（38 种非没食子酰化和 34 种没食子酰化）和 53 种（24 种原花青素和 29 种原矢车菊素）黄烷醇。对详细黄烷醇组成的 HCA 和 PCA 分析允许区分 Rufete 克隆。
