Yasuda J, Tateyama K, Syuto B, Too K
Veterinary Hospital, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Jpn J Vet Res. 1990 Jan;38(1):19-29.
The lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) isoenzyme distributions in tissues of the ICR mouse, Wistar rat, guinea pig and golden hamster were analyzed by histoelectrophoresis. Tissues obtained were as follows: liver, pancreas, stomach, small intestine, heart, femoral muscle, uterus, kidney, spleen, lymph node, cerebrum, spinal cord and erythrocyte. Histoelectrophoresis was for the direct analysis of LDH and CPK isoenzymes in the tissues and had high practical value compared with previous tissue-extraction methods. In tissues of the mouse, guinea pig and golden hamster, LDH isoenzymes showed five bands. In the rat, LDH isoenzyme was separated into four fractions. CPK isoenzyme showed three bands; BB, MB and MM. In some tissues, the MM band was separated into two sub fractions.
采用组织电泳法分析了ICR小鼠、Wistar大鼠、豚鼠和金黄仓鼠组织中的乳酸脱氢酶（LDH）和肌酸磷酸激酶（CPK）同工酶分布。获取的组织如下：肝脏、胰腺、胃、小肠、心脏、股肌、子宫、肾脏、脾脏、淋巴结、大脑、脊髓和红细胞。组织电泳用于直接分析组织中的LDH和CPK同工酶，与以往的组织提取方法相比具有较高的实用价值。在小鼠、豚鼠和金黄仓鼠的组织中，LDH同工酶呈现出五条带。在大鼠中，LDH同工酶被分离为四个组分。CPK同工酶呈现出三条带：BB、MB和MM。在某些组织中，MM带被分离为两个亚组分。
