Sun Hao, Ni Xueqin, Zeng Dong, Zou Fuqin, Yang Mingyue, Peng Zhirong, Zhou Yi, Zeng Yan, Zhu Hui, Wang Hesong, Yin Zhongqiong, Pan Kangcheng, Jing Bo
Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, No. 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, China.
Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, No. 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, China.
Res Vet Sci. 2017 Feb;110:22-28. doi: 10.1016/j.rvsc.2016.10.015. Epub 2016 Oct 27.
Accumulating evidence has proven the immunomodulating activity of Yupingfeng. This study compared the immunomodulatory activity in vitro of the unfermented Yupingfeng dreg polysaccharides (UYDP) with that of the fermented Yupingfeng dreg polysaccharides (FYDP) obtained using Rhizopus oligosporus SH. Results consistently elucidated the duality of the immunomodulatory roles of UYDP and FYDP in regulating proliferation, and cytokines expressions in murine lymphocytes and macrophages. Compared with UYDP, FYDP effectively enhanced the proliferation of lymphocytes and promoted mRNA expression of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, INF-γ, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and TLR-4 in lymphocytes and macrophages. Moreover, compared with UYDP, FYDP effectively normalized cell proliferation and downregulated mRNA expression levels of pro-inflammatory cytokines, NF-κB, TLR-4, and iNOs in lipopolysaccharide-induced chronic inflammation cells. The results revealed that the bidirectional immunomodulatory effects in vitro of UYDP and FYDP, and the bi-directional immunomodulatory activity of FYDP is superior over that of UYDP. Moreover, more studies in vivo that needs to be studied further.
越来越多的证据证明了玉屏风的免疫调节活性。本研究比较了未发酵的玉屏风渣多糖（UYDP）与使用少孢根霉SH获得的发酵玉屏风渣多糖（FYDP）在体外的免疫调节活性。结果一致阐明了UYDP和FYDP在调节小鼠淋巴细胞和巨噬细胞增殖及细胞因子表达方面免疫调节作用的双重性。与UYDP相比，FYDP有效增强了淋巴细胞的增殖，并促进了淋巴细胞和巨噬细胞中诱导型一氧化氮合酶（iNOS）、IL-6、TNF-α、INF-γ、活化B细胞核因子κB（NF-κB）和TLR-4的mRNA表达。此外，与UYDP相比，FYDP有效使细胞增殖正常化，并下调脂多糖诱导的慢性炎症细胞中促炎细胞因子、NF-κB、TLR-4和iNOs的mRNA表达水平。结果表明，UYDP和FYDP在体外具有双向免疫调节作用，且FYDP的双向免疫调节活性优于UYDP。此外，还需要进一步开展更多的体内研究。
