College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Int J Biol Macromol. 2018 Mar;108:300-306. doi: 10.1016/j.ijbiomac.2017.12.025. Epub 2017 Dec 6.
Polysaccharides from Collybia radicata mushroom (CRP) possess many functions, such as antiviral, anti-aging and hypolipidemic activities. However, little is known about their immunomodulatory activity. To address this issue, we did a thorough research into their immune effects on murine macrophages. The results showed that the 14942Da polysaccharide not only obviously improved the proliferation and phagocytosis of macrophages, but also induced the secretion of nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin factors (IL-1β, IL-6 and IL-10). At a concentration of 850.0μgmL, the polysaccharide stimulated their proliferation and phagocytosis to 2.1 and 3.4 times, respectively, as compared to the negative group. Meanwhile, it raised the production of NO by inducing iNOS in a concentration-dependent manner. Furthermore, it enhanced the release of these cytokines to multiples from 2.3 to 3.6 times. As an inhibitor of TLR4 (Toll-like Receptor 4), TAK242 suppressed the secretion of NO, iNOS and cytokines above 51%, and ORP acted on the cells mainly via TLR4. Consequently, the polysaccharide has a potent immunomodulatory activity by stimulating macrophages and can be considered as a novel potential immunopotentiator in medical and food industries.
糙皮侧耳多糖（CRP）具有抗病毒、抗衰老、降血脂等多种功能。但关于其免疫调节活性的研究还很少。为解决这一问题，我们深入研究了糙皮侧耳多糖对小鼠巨噬细胞的免疫作用。结果表明，14942Da 多糖不仅明显促进了巨噬细胞的增殖和吞噬作用，而且诱导了一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）和细胞因子（如肿瘤坏死因子-α（TNF-α）和白细胞介素因子（IL-1β、IL-6 和 IL-10）的分泌。在 850.0μgmL 的浓度下，多糖刺激其增殖和吞噬作用分别达到阴性组的 2.1 倍和 3.4 倍。同时，它通过诱导 iNOS 以浓度依赖的方式增加 NO 的产生。此外，它将这些细胞因子的释放提高到 2.3 至 3.6 倍。作为 TLR4（Toll-like Receptor 4）的抑制剂，TAK242 抑制 NO、iNOS 和细胞因子的分泌超过 51%，而 ORP 主要通过 TLR4 作用于细胞。因此，多糖通过刺激巨噬细胞具有很强的免疫调节活性，可以被认为是医学和食品工业中一种新型的潜在免疫增强剂。
