Guan Yuling, Zheng Wendan, Bai Yu, Wu Bo
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, China.
Front Vet Sci. 2024 Feb 23;11:1337698. doi: 10.3389/fvets.2024.1337698. eCollection 2024.
polysaccharide (YPF-P) is the main substance of alcohol deposition in powder, which has many biological functions such as enhancing immunity, repairing intestinal barrier and enhancing antioxidant ability. This study employed growth-promoting drug feed additives and animal experiments to comprehensively evaluate the use of YPF-P in broiler production.
A total of 1,296 151 days-old Partridge chickens were randomly divided into four groups with six replicates and 54 hens per replicate: the control group was fed basal diet, and the experimental groups were fed diets supplemented with 4 g/kg, 8 g/kg, and 12 g/kg YPF-P for 14 days. Broilers were weighed before and at the end of the experiment to calculate total weight gain (GW), average daily gain (ADG), and feed compensation. At the end of the experiment, six chickens from each group were randomly selected for subwing vein blood sampling, which was used to measure serum biochemical indicators GHRH, GH, and IGF-1 by ELISA method. Randomly select chickens from control group and 8 g/kg group for slaughter, and cecal contents were collected for 16S high-throughput sequencing.
Dietary supplementation of 8 g/kg YPF-P can significantly increase the final body weight, total weight gain, average daily gain and decrease the feed to gain ratio of chickens. During 151-165 days, serum IGF-1 concentrations increased significantly ( < 0.05). There were no significant changes in serum GH concentration ( > 0.05). In terms of gut microbiota, there was no significant difference between control group and test group in Shannon index and Simpson index. Compared with the control group,the addition of 8 g/kgYPF-P significantly increased the abundance of and significantly decreased the abundance of Bacteroides at the phylum level.At the genus level, the relative abundance of was significantly increased and the , , , , , , and was significantly decreased.
The above results showed that adding 8 mg/kg of YPF-P could increase the average daily gain of Partridge chickens, reduce the ratio of feed to meat, and affect the distribution proportion of intestinal microflora in chickens to some extent.
多糖（YPF-P）是粉剂中酒精沉淀的主要物质，具有增强免疫力、修复肠道屏障和增强抗氧化能力等多种生物学功能。本研究采用促生长药物饲料添加剂和动物实验，对YPF-P在肉鸡生产中的应用进行综合评价。
选取1296只15日龄的鹧鸪鸡，随机分为4组，每组6个重复，每个重复54只母鸡。对照组饲喂基础日粮，实验组分别饲喂添加4g/kg、8g/kg和12g/kg YPF-P的日粮，持续14天。在实验开始前和结束时对肉鸡进行称重，计算总增重（GW）、平均日增重（ADG）和饲料报酬。实验结束时，每组随机选取6只鸡进行翅下静脉采血，采用ELISA法测定血清生化指标生长激素释放激素（GHRH）、生长激素（GH）和胰岛素样生长因子-1（IGF-1）。从对照组和8g/kg组中随机选取鸡进行屠宰，采集盲肠内容物进行16S高通量测序。
日粮中添加8g/kg YPF-P可显著提高鸡的末体重、总增重、平均日增重，并降低料肉比。在15-165日龄期间，血清IGF-1浓度显著升高（P<0.05）。血清GH浓度无显著变化（P>0.05）。在肠道微生物群方面，对照组和试验组的香农指数和辛普森指数无显著差异。与对照组相比，添加8g/kg YPF-P显著增加了门水平上拟杆菌门的丰度，显著降低了拟杆菌属的丰度。在属水平上，双歧杆菌属的相对丰度显著增加，而肠杆菌属、大肠杆菌属、乳杆菌属、梭菌属、葡萄球菌属、肠球菌属和弯曲杆菌属的相对丰度显著降低。
上述结果表明，添加8mg/kg YPF-P可提高鹧鸪鸡的平均日增重，降低料肉比，并在一定程度上影响鸡肠道微生物群落的分布比例。
