Šulniūtė Vaida, Pukalskas Audrius, Venskutonis Petras Rimantas
Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, LT-50254, Lithuania.
Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, LT-50254, Lithuania.
Food Chem. 2017 Jun 1;224:37-47. doi: 10.1016/j.foodchem.2016.12.047. Epub 2016 Dec 18.
Ten Salvia species, S. amplexicaulis, S. austriaca, S. forsskaolii S. glutinosa, S. nemorosa, S. officinalis, S. pratensis, S. sclarea, S. stepposa and S. verticillata were fractionated using supercritical carbon dioxide and pressurized liquid (ethanol and water) extractions. Fifteen phytochemicals were identified using commercial standards (some other compounds were identified tentatively), 11 of them were quantified by ultra high pressure chromatography (UPLC) with quadruple and time-of-flight mass spectrometry (Q/TOF, TQ-S). Lipophilic CO extracts were rich in tocopherols (2.36-10.07mg/g), while rosmarinic acid was dominating compound (up to 30mg/g) in ethanolic extracts. Apigenin-7-O-β-d-glucuronide, caffeic and carnosic acids were quantitatively important phytochemicals in the majority other Salvia spp. Antioxidatively active constituents were determined by using on-line high-performance liquid chromatography (HPLC) analysis combined with 2,2'-diphenyl-1-picrylhydrazyl (DPPH) assay (HPLC-DPPH). Development of high pressure isolation process and comprehensive characterisation of phytochemicals in Salvia spp. may serve for their wider applications in functional foods and nutraceuticals.
使用超临界二氧化碳和加压液体（乙醇和水）萃取法对10种鼠尾草属植物进行了分离，这10种植物分别是抱茎鼠尾草、奥地利鼠尾草、福氏鼠尾草、粘毛鼠尾草、林地鼠尾草、药用鼠尾草、草原鼠尾草、南欧丹参、草原鼠尾草和轮叶鼠尾草。使用商业标准鉴定了15种植物化学物质（其他一些化合物为初步鉴定），其中11种通过超高压色谱（UPLC）结合四极杆和飞行时间质谱（Q/TOF，TQ-S）进行了定量分析。亲脂性CO提取物富含生育酚（2.36 - 10.07mg/g），而迷迭香酸是乙醇提取物中的主要化合物（含量高达30mg/g）。芹菜素-7-O-β-D-葡萄糖醛酸、咖啡酸和肌醇六磷酸在大多数其他鼠尾草属植物中是重要的定量植物化学物质。通过在线高效液相色谱（HPLC）分析结合2,2'-二苯基-1-苦基肼（DPPH）测定法（HPLC-DPPH）测定抗氧化活性成分。鼠尾草属植物高压分离工艺的开发和植物化学物质的综合表征可能有助于它们在功能性食品和营养保健品中的更广泛应用。
